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SPARCL1 Polyclonal antibody, PBS Only (13517-1-PBS)

Filter: AllWBIPIHC

WB analysis of human heart using 13517-1-AP (same clone as 13517-1-PBS)
human heart tissue were subjected to SDS PAGE followed by western blot with 13517-1-AP (SPARCL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13517-1-PBS in a different storage buffer formulation.
IP experiment of Raji using 13517-1-AP (same clone as 13517-1-PBS)
IP result of anti-SPARCL1 (IP:13517-1-AP, 4ug; Detection:13517-1-AP 1:300) with Raji cells lysate 1440ug. This data was developed using the same antibody clone with 13517-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 13517-1-AP (same clone as 13517-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13517-1-AP (SPARCL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13517-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 13517-1-AP (same clone as 13517-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13517-1-AP (SPARCL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13517-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 13517-1-AP (same clone as 13517-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13517-1-AP (SPARCL1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13517-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 13517-1-AP (same clone as 13517-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13517-1-AP (SPARCL1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13517-1-PBS in a different storage buffer formulation.

SPARCL1 Polyclonal antibody, PBS Only

Name: SPARCL1 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 13517-1-PBS

SPARCL1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 13517-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

13517-1-PBS targets SPARCL1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4448 Product name: Recombinant human SPARCL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 316-664 aa of BC033721 Sequence: VSEALLMEPTDDGNTTPRNHGVDDDGDDDGDDGGTDGPRHSASDDYFIPSQAFLEAERAQSIAYHLKIEEQREKVHENENIGTTEPGEHQEAKKAENSSNEEETSSEGNMRVHAVDSCMSFQCKRGHICKADQQGKPHCVCQDPVTCPPTKPLDQVCGTDNQTYASSCHLFATKCRLEGTKKGHQLQLDYFGACKSIPTCTDFEVIQFPLRMRDWLKNILMQLYEANSEHAGYLNEKQRNKVKKIYLDEKRLLAGDHPIDLLLRDFKKNYHMYVYPVHWQFSELDQHPMDRVLTHSELAPLRASLVPMEHCITRFFEECDPNKDKHITLKEWGHCFGIKEEDIDENLLF Predict reactive species
Full Name	SPARC-like 1 (hevin)
Calculated Molecular Weight	664 aa, 75 kDa
Observed Molecular Weight	130-140 kDa
GenBank Accession Number	BC033721
Gene Symbol	SPARCL1
Gene ID (NCBI)	8404
RRID	AB_10598475
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14515
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SPARC-like protein 1 (SPARCL1), also known as SC1 or Hevin, is an extracellular matrix glycoprotein that belongs to the SPARC family. SPARCL1 is widely expressed in normal and cancer tissues, and has been shown to regulate cellular adhesion and migration. SPARCL1 has been implicated in tumor initiation, formation, and progression of various cancers, including pancreatic, prostate, bladder, ovarian, breast and non-small cell lung cancers.