SPATA16 Polyclonal antibody

SPATA16 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 17357-1-AP

Synonyms

NYD SP12, SPATA16, spermatogenesis associated 16

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Validation Data Gallery

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  IHC staining of human testis using 17357-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17357-1-AP (SPATA16 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 17357-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17357-1-AP (SPATA16 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17357-1-AP targets SPATA16 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPATA16 fusion protein Ag10637
• Full Name: spermatogenesis associated 16
• Calculated Molecular Weight: 569 aa, 65 kDa
• GenBank Accession Number: BC034496
• Gene Symbol: SPATA16
• Gene ID (NCBI): 83893
• RRID: AB_2878396
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPATA16, also named as NYD-SP12, belongs to the SPATA16 family. It is involved in the formation of sperm acrosome, which implicated its potential role in spermatogenesis and sperm-egg fusion. SPATA16 localizes to Golgi apparatus and is primarily expressed in testis, with lower levels found in kidney and pancreas.