SPATA2 Polyclonal antibody

SPATA2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11000-1-AP

Synonyms

KIAA0757, SPATA2, spermatogenesis associated 2, tamo

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Validation Data Gallery

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  WB analysis of human lung using 11000-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11000-1-AP (SPATA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 11000-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11000-1-AP (SPATA2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse lung using 11000-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11000-1-AP (SPATA2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse lung using 11000-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11000-1-AP (SPATA2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: human lung tissue
• Positive IHC detected in: human prostate cancer tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11000-1-AP targets SPATA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPATA2 fusion protein Ag1432
• Full Name: spermatogenesis associated 2
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC009481
• Gene Symbol: SPATA2
• Gene ID (NCBI): 9825
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	PLoS Genet; Pharmacological vitamin C inhibits mTOR signaling and tumor growth by degrading Rictor and inducing HMOX1 expression; Authors - Senlin Qin