SPATA2 Polyclonal antibody

SPATA2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29321-1-AP

Synonyms

FLJ13167, KIAA0757, PD1, SPATA2, spermatogenesis associated 2, tamo

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Validation Data Gallery

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  WB analysis using 29321-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29321-1-AP (SPATA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 29321-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29321-1-AP (SPATA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 29321-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29321-1-AP (SPATA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 29321-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SPATA2 antibody (29321-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IHC detected in: mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29321-1-AP targets SPATA2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPATA2 fusion protein Ag30773
• Full Name: spermatogenesis associated 2
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58-60 kDa
• GenBank Accession Number: BC009481
• Gene Symbol: SPATA2
• Gene ID (NCBI): 9825
• RRID: AB_2918282
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The protein SPATA2 (spermatogenesis-associated protein 2) was initially shown to be highly expressed in testicular Sertoli cells, and these early reports suggested SPATA2 to play a role in spermatogenesis. More recently, it was discussed to be involved in the predisposition for psoriasis. SPATA2 is required for TNF-induced cell death. SPATA2 has a potential role during pancreatic development and β-cell proliferation.(PMID: 20119533/PMID: 27458237)