SPATS2L Polyclonal antibody

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• SPATS2L Polyclonal antibody (16938-1-AP)

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• KD/KO Validated

SPATS2L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16938-1-AP Copied!

Publications (4)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

LOC26010, SPATS2 like protein, SP1224, SGNP, DNAPTP6

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  WB analysis of HepG2 using 16938-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16938-1-AP (SPATS2L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 16938-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16938-1-AP (SPATS2L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human spleen using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human spleen using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 16938-1-AP

  Immunofluorescent analysis of HepG2 cells, using LOC26010 antibody 16938-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of Saos-2 using 16938-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using SPATS2L antibody (16938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse kidney tissue
• Positive IHC detected in: human kidney tissue, human heart tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Saos-2 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16938-1-AP targets SPATS2L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10438

  Product name: Recombinant human SPATS2L protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 134-488 aa of BC018736

  Sequence: SILEEPSKALRGVTGPNIEKSVKDLQRCTVSLTRYRVMIKEEVDSSVKKIKAAFAELHNCIIDKEVSLMAEMDKVKEEAMEILTARQKKAEELKRLTDLASQMAEMQLAELRAEIKHFVSERKYDEELGKAARFSCDIEQLKAQIMLCGEITHPKNNYSSRTPCSSLLPLLNAHAATSGKQSNFSRKSSTHNKPSEGKAANPKMVSSLPSTADPSHQTMPANKQNGSSNQRRRFNPQYHNNRLNGPAKSQGSGNEAEPLGKGNSRHEHRRQPHNGFRPKNKGGAKNQEASLGMKTPEAPAHSEKPRRRQHAADTSEARPFRGSVGRVSQCNLCPTRIEVSTDAAVLSVPAVTLVA

• Full Name: viral DNA polymerase-transactivated protein 6
• Calculated Molecular Weight: 488 aa, 54 kDa
• Observed Molecular Weight: 54-62 kDa
• GenBank Accession Number: BC018736
• Gene Symbol: SPATS2L
• Gene ID (NCBI): 26010
• RRID: AB_1851075
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NUQ6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPATS2L encodes spermatogenesis associated and serine-rich 2-like protein, also named DNAPTP6 (DNA polymerase transactivated protein 6) in human (PMID:11230166). SPATS2L acts as an important regulator of β2-adrenergic receptor down-regulation (PubMed: 25562107). MiR-1269a down-regulates the expression of SPATS2L and LRP6, thereby inhibiting the proliferation of liver cancer cells (PMID: 28081866, PMID:35252180). SPATS2L has several isoforms by alternative splicing.

Publications

What published studies show

The SPATS2L Polyclonal antibody (Cat# 16938-1-AP) has 4 citations. These appear in Science Advances, Journal of Translational Medicine, Frontiers in Cell and Developmental Biology, and Scientific Reports. The research fields span stress granule assembly in neurons, acute myeloid leukemia (JAK2/STAT3/STAT5 axis), glioma survival prediction via RNA-binding protein signatures, and DNA methylation profiles in recurrent miscarriage. Applications cited include WB, IHC, and IF across human and mouse species.

Automatically generated from the 4 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Sci Adv; RNA G-quadruplex organizes stress granule assembly through DNAPTP6 in neurons; Authors - Sefan Asamitsu
human	WB	J Transl Med; Spermatogenesis associated serine rich 2 like plays a prognostic factor and therapeutic target in acute myeloid leukemia by regulating the JAK2/STAT3/STAT5 axis; Authors - Fenglin Li ; KD Validated
human	WB	Front Cell Dev Biol; A Novel Signature Constructed by RNA-Binding Protein Coding Genes to Improve Overall Survival Prediction of Glioma Patients.; Authors - Zewei Tu
human	IHC,WB	Sci Rep; Characteristic DNA methylation profiles of chorionic villi in recurrent miscarriage.; Authors - Yosuke Matsumoto ; KD Validated