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SPATS2L Polyclonal antibody, PBS Only (29930-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis using 29930-1-AP (same clone as 29930-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 29930-1-AP (SPATS2L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.
WB analysis using 29930-1-AP (same clone as 29930-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 29930-1-AP (SPATS2L antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 29930-1-AP (same clone as 29930-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29930-1-AP (SPATS2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 29930-1-AP (same clone as 29930-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29930-1-AP (SPATS2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 29930-1-AP (same clone as 29930-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29930-1-AP (SPATS2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 29930-1-AP (same clone as 29930-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29930-1-AP (SPATS2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 29930-1-AP (same clone as 29930-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SPATS2L antibody (29930-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 29930-1-AP (same clone as 29930-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SPATS2L antibody (29930-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 29930-1-AP (same clone as 29930-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SPATS2L antibody (29930-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

SPATS2L Polyclonal antibody, PBS Only

Name: SPATS2L Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 29930-1-PBS

SPATS2L Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 29930-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

29930-1-PBS targets SPATS2L in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag31157 Product name: Recombinant human SPATS2L protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 290-488 aa of BC018736 Sequence: NYSSRTPCSSLLPLLNAHAATSGKQSNFSRKSSTHNKPSEGKAANPKMVSSLPSTADPSHQTMPANKQNGSSNQRRRFNPQYHNNRLNGPAKSQGSGNEAEPLGKGNSRHEHRRQPHNGFRPKNKGGAKNQEASLGMKTPEAPAHSEKPRRRQHAADTSEARPFRGSVGRVSQCNLCPTRIEVSTDAAVLSVPAVTLVA Predict reactive species
Full Name	viral DNA polymerase-transactivated protein 6
Calculated Molecular Weight	488 aa, 54 kDa
Observed Molecular Weight	54-65 kDa
GenBank Accession Number	BC018736
Gene Symbol	SPATS2L
Gene ID (NCBI)	26010
RRID	AB_2923620
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NUQ6
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SPATS2L encodes spermatogenesis associated and serine-rich 2-like protein, also named DNAPTP6 (DNA polymerase transactivated protein 6) in human (PMID:11230166). SPATS2L acts as an important regulator of β2-adrenergic receptor down-regulation (PubMed: 25562107). MiR-1269a down-regulates the expression of SPATS2L and LRP6, thereby inhibiting the proliferation of liver cancer cells (PMID: 28081866, PMID:35252180). SPATS2L has several isoforms by alternative splicing.