Tested Applications
| Positive WB detected in | SKOV-3 cells, THP-1 cells |
| Positive IHC detected in | mouse stomach tissue, mouse kidney tissue, rat stomach tissue, rat kidney tissue, human ovary cancer tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26474-1-AP targets SPC25 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23985 Product name: Recombinant human SPC25 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-224 aa of BC022255 Sequence: MVEDELALFDKSINEFWNKFKSTDTSCQMAGLRDTYKDSIKAFAEKLSVKLKEEERMVEMFLEYQNQISRQNKLIQEKKDNLLKLIAEVKGKKQELEVLTANIQDLKEEYSRKKETISTANKANAERLKRLQKSADLYKDRLGLEIRKIYGEKLQFIFTNIDPKNPESPFMFSLHLNEARDYEVSDSAPHLEGLAEFQENVRKTNNFSAFLANVRKAFTATVYN Predict reactive species |
| Full Name | SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae) |
| Observed Molecular Weight | 26-30 kDa |
| GenBank Accession Number | BC022255 |
| Gene Symbol | SPC25 |
| Gene ID (NCBI) | 57405 |
| RRID | AB_2880530 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HBM1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Spindle Pole Body Component 25 (SPC25), a core subunit of the NDC80 kinetochore complex, is essential for accurate chromosome alignment and segregation during mitosis . By linking microtubules to kinetochores, SPC25 ensures proper attachment and tension generation, critical for the maintenance of genomic stability . SPC25 is largely found in the cytoplasm and nucleus. The NDC80 complex, which is involved in kinetochore-microtubule interactions and spindle assembly checkpoint activity, is composed of four subunits: SPC24, SPC25, NDC80, and NUF2. (PMID: 40587622 ,PMID: 40400636)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SPC25 antibody 26474-1-AP | Download protocol |
| IHC protocol for SPC25 antibody 26474-1-AP | Download protocol |
| WB protocol for SPC25 antibody 26474-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SPC25 Polyclonal antibody (Cat# 26474-1-AP) has 5 citations published in Aging (Albany NY), Cancers (Basel), Expert Review of Clinical Immunology, JTO Clinical Research Reports, and Cell Biology International. Research fields include hepatocellular carcinoma prognosis, lung adenocarcinoma progression, tumor proliferation, glutamine metabolism, anti-tumor NK cell immunity, idiopathic pulmonary fibrosis, and PI3K/AKT signaling pathway. Cited applications include Western blot and immunohistochemistry in human samples.
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) SPC25 overexpression promotes tumor proliferation and is prognostic of poor survival in hepatocellular carcinoma. | ||
Cancers (Basel) Integrating Functional Genomic Screens and Multi-Omics Data to Construct a Prognostic Model for Lung Adenocarcinoma and Validating SPC25.
| ||
Expert Rev Clin Immunol TFDP1 activates SPC25-mediated glutamine metabolism to repress anti-tumor immunity of NK cells in lung adenocarcinoma | ||
JTO Clin Res Rep CADM1 and SPC25 Gene Mutations in Lung Cancer Patients With Idiopathic Pulmonary Fibrosis.
| ||
Cell Biol Int PLEK2 activates the PI3K/AKT signaling pathway to drive lung adenocarcinoma progression by upregulating SPC25 |
