SPC25 Polyclonal antibody

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  WB analysis using 26474-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26474-1-AP (SPC25 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 26474-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26474-1-AP (SPC25 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 26474-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26474-1-AP (SPC25 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

SPC25 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26474-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

AD024, hSpc25, Kinetochore protein Spc25, SPBC25

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Tested Applications

• Positive WB detected in: SKOV-3 cells, THP-1 cells
• Positive IHC detected in: mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

26474-1-AP targets SPC25 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23985

  Product name: Recombinant human SPC25 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-224 aa of BC022255

  Sequence: MVEDELALFDKSINEFWNKFKSTDTSCQMAGLRDTYKDSIKAFAEKLSVKLKEEERMVEMFLEYQNQISRQNKLIQEKKDNLLKLIAEVKGKKQELEVLTANIQDLKEEYSRKKETISTANKANAERLKRLQKSADLYKDRLGLEIRKIYGEKLQFIFTNIDPKNPESPFMFSLHLNEARDYEVSDSAPHLEGLAEFQENVRKTNNFSAFLANVRKAFTATVYN

• Full Name: SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae)
• Observed Molecular Weight: 26-30 kDa
• GenBank Accession Number: BC022255
• Gene Symbol: SPC25
• Gene ID (NCBI): 57405
• RRID: AB_2880530
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HBM1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Aging (Albany NY); SPC25 overexpression promotes tumor proliferation and is prognostic of poor survival in hepatocellular carcinoma.; Authors - Baozhu Zhang
human	WB	Cell Biol Int; PLEK2 activates the PI3K/AKT signaling pathway to drive lung adenocarcinoma progression by upregulating SPC25; Authors - Wenqian Zhang
human	WB,IHC	JTO Clin Res Rep; CADM1 and SPC25 Gene Mutations in Lung Cancer Patients With Idiopathic Pulmonary Fibrosis.; Authors - Aya Fukuizumi ; KD Validated