SPCS3 Polyclonal antibody

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  WB analysis using 17640-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17640-1-AP (SPCS3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat liver using 17640-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17640-1-AP (SPCS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 17640-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17640-1-AP (SPCS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SPCS3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17640-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

PRO3567, SPase 22/23 kDa subunit, SPC22, SPC22/23, SPC3, SPCS3, YLR066W

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Tested Applications

• Positive WB detected in: HeLa cells, U2OS cells, mouse kidney tissue, mouse liver tissue
• Positive IHC detected in: human placenta tissue, rat liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17640-1-AP targets SPCS3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPCS3 fusion protein Ag11780
• Full Name: signal peptidase complex subunit 3 homolog (S. cerevisiae)
• Calculated Molecular Weight: 180 aa, 20 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC047290
• Gene Symbol: SPCS3
• Gene ID (NCBI): 60559
• RRID: AB_3669279
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P61009
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The microsomal signal peptidase complex (SPC), which is an essential membrane component in the endoplasmic reticulum (ER), has five subunits, including SEC11A, SEC11C, SPCS1, SPCS2, and SPCS3. Functionally, SPC removes signal peptides (SPs) from a series of secretory pre-proteins with high specificity. The dysregulation of the other SPC subunits was also observed in some tumors. SPCS3 expression can be used as a component of a risk score model to predict the survival of patients with pancreatic adenocarcinoma. (PMID: 35653344)