SPCS3 Polyclonal antibody, PBS Only

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• SPCS3 Polyclonal antibody, PBS Only (17640-1-PBS)

• 

  WB analysis using 17640-1-AP (same clone as 17640-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17640-1-AP (SPCS3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17640-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 17640-1-AP (same clone as 17640-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17640-1-AP (SPCS3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17640-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 17640-1-AP (same clone as 17640-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17640-1-AP (SPCS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17640-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 17640-1-AP (same clone as 17640-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17640-1-AP (SPCS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17640-1-PBS in a different storage buffer formulation.

SPCS3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 17640-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

PRO3567, SPase 22/23 kDa subunit, SPC22, SPC22/23, SPC3

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Product Information

17640-1-PBS targets SPCS3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11780

  Product name: Recombinant human SPCS3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-180 aa of BC047290

  Sequence: MNTVLSRANSLFAFSLSVMAALTFGCFITTAFKDRSVPVRLHVSRIMLKNVEDFTGPRERSDLGFITFDITADLENIFDWNVKQLFLYLSAEYSTKNNALNQVVLWDKIVLRGDNPKLLLKDMKTKYFFFDDGNGLKGNRNVTLTLSWNVVPNAGILPLVTGSGHVSVPFPDTYEITKSY

• Full Name: signal peptidase complex subunit 3 homolog (S. cerevisiae)
• Calculated Molecular Weight: 180 aa, 20 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC047290
• Gene Symbol: SPCS3
• Gene ID (NCBI): 60559
• RRID: AB_3669279
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P61009
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The microsomal signal peptidase complex (SPC), which is an essential membrane component in the endoplasmic reticulum (ER), has five subunits, including SEC11A, SEC11C, SPCS1, SPCS2, and SPCS3. Functionally, SPC removes signal peptides (SPs) from a series of secretory pre-proteins with high specificity. The dysregulation of the other SPC subunits was also observed in some tumors. SPCS3 expression can be used as a component of a risk score model to predict the survival of patients with pancreatic adenocarcinoma. (PMID: 35653344)