SPDYC Polyclonal antibody
SPDYC Polyclonal Antibody for IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IHC, ELISA
Conjugate
Unconjugated
Cat no : 22471-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human liver tissue, human kidney tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
22471-1-AP targets SPDYC in IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SPDYC fusion protein Ag18126 |
Full Name | speedy homolog C (Xenopus laevis) |
Calculated Molecular Weight | 293 aa, 33 kDa |
Observed Molecular Weight | 33-40 kDa |
GenBank Accession Number | BC137244 |
Gene Symbol | SPDYC |
Gene ID (NCBI) | 387778 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen Affinity purified |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SPDYC(Speedy protein C), also known as RINGOC and Ringo2, promotes progression through the cell cycle via binding and activation of CDK1 and CDK2. Downregulation of SPDYC inhibits cell growth, reduces the fraction of cells in G1, and increases the S and G2 populations(PMID: 18802405). SPDYC is involved in the spindle-assembly checkpoint and required for recruitment of MAD2L1, BUBR1 and BUB1 to kinetochores(Uniprot).
Protocols
Product Specific Protocols | |
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IHC protocol for SPDYC antibody 22471-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |