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SPDYC Polyclonal antibody, PBS Only (22471-1-PBS)

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IHC staining of human liver using 22471-1-AP (same clone as 22471-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22471-1-AP (SPDYC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22471-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 22471-1-AP (same clone as 22471-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22471-1-AP (SPDYC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22471-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 22471-1-AP (same clone as 22471-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22471-1-AP (SPDYC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22471-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 22471-1-AP (same clone as 22471-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22471-1-AP (SPDYC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22471-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 22471-1-AP (same clone as 22471-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22471-1-AP (SPDYC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22471-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 22471-1-AP (same clone as 22471-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22471-1-AP (SPDYC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22471-1-PBS in a different storage buffer formulation.

SPDYC Polyclonal antibody, PBS Only

SPDYC Polyclonal Antibody for IHC, Indirect ELISA

Cat No. 22471-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, Indirect ELISA

Synonyms

hSpy/Ringo C, RINGO C, Ringo2, Speedy protein C

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

22471-1-PBS targets SPDYC in IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag18126 Product name: Recombinant human SPDYC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-293 aa of BC137244 Sequence: MLWAIPELGSPCPISISYEMSDSQDPTTSPVVTTQVELGGCSRQGGGNGFLRFRQHQEVQAFLSLLEDSFVQEFLSKDPCFQISDKYLLAMVLVYFQRAHLKLSEYTHSSLFLALYLANDMEEDLEGPKCEIFPWALGKDWCLRVGKFLHQRDKLWARMGFRAVVSRQCCEEVMAKEPFHWAWTRDRRPHHGGVQRVCPQVPVRLPRGPGLSPPHCSPCGLPQHCSSHLLKPVSSKCPSLTSECHRPPSQNYLSRVKNAWGGDFLIVLPPQMQLEPGTYSLRIFPKPPARPGH Predict reactive species
Full Name	speedy homolog C (Xenopus laevis)
Calculated Molecular Weight	293 aa, 33 kDa
Observed Molecular Weight	33-40 kDa
GenBank Accession Number	BC137244
Gene Symbol	SPDYC
Gene ID (NCBI)	387778
RRID	AB_3085699
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
UNIPROT ID	Q5MJ68
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SPDYC(Speedy protein C), also known as RINGOC and Ringo2, promotes progression through the cell cycle via binding and activation of CDK1 and CDK2. Downregulation of SPDYC inhibits cell growth, reduces the fraction of cells in G1, and increases the S and G2 populations(PMID: 18802405). SPDYC is involved in the spindle-assembly checkpoint and required for recruitment of MAD2L1, BUBR1 and BUB1 to kinetochores(Uniprot).