Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28010-1-AP targets SPESP1 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27764 Product name: Recombinant human SPESP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 139-344 aa of BC017998 Sequence: YIENEEPEPEPEPAAKQTEAPRMLPVVTESSTSPYVTSYKSPVTTLDKSTGIGISTESEDVPQLSGETAIEKPEEFGKHPESWNNDDILKKILDINSQVQQALLSDTSNPAYREDIEASKDHLKRSLALAAAAEHKLKTMYKSQLLPVGRTSNKIDDIETVINMLCNSRSKLYEYLDIKCVPPEMREKAATVFNTLKNMCRSRRVT Predict reactive species |
| Full Name | sperm equatorial segment protein 1 |
| Calculated Molecular Weight | 39 kDa |
| Observed Molecular Weight | 67 kDa, 77 kDa |
| GenBank Accession Number | BC017998 |
| Gene Symbol | SPESP1 |
| Gene ID (NCBI) | 246777 |
| RRID | AB_2881036 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6UW49 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SPESP1 antibody 28010-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SPESP1 Polyclonal antibody (Cat# 28010-1-AP) has 1 citation to date. It was cited in Clinical and Translational Medicine (IF 7.9), in a 2024 study investigating how downregulated SPESP1-driven fibroblast senescence impairs wound healing in aged mice. The research falls within the fields of aging biology, fibroblast senescence, and wound repair. The antibody was applied in Western blot and immunofluorescence for both human and mouse samples.
| Species | Application | Title |
|---|---|---|
Clin Transl Med Downregulated SPESP1-driven fibroblast senescence decreases wound healing in aged mice
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