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SPESP1 Polyclonal antibody

SPESP1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF, ELISA

Equatorial segment protein, ESP, SP ESP, SPESP1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 
SKU: 

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Quantity

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What's the best dilution for SPESP1 in ?

Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28010-1-AP targets SPESP1 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag27764

Product name: Recombinant human SPESP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 139-344 aa of BC017998

Sequence: YIENEEPEPEPEPAAKQTEAPRMLPVVTESSTSPYVTSYKSPVTTLDKSTGIGISTESEDVPQLSGETAIEKPEEFGKHPESWNNDDILKKILDINSQVQQALLSDTSNPAYREDIEASKDHLKRSLALAAAAEHKLKTMYKSQLLPVGRTSNKIDDIETVINMLCNSRSKLYEYLDIKCVPPEMREKAATVFNTLKNMCRSRRVT

Predict reactive species
Full Name sperm equatorial segment protein 1
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 67 kDa, 77 kDa
GenBank Accession NumberBC017998
Gene Symbol SPESP1
Gene ID (NCBI) 246777
RRIDAB_2881036
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UW49
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SPESP1 antibody 28010-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The SPESP1 Polyclonal antibody (Cat# 28010-1-AP) has 1 citation to date. It was cited in Clinical and Translational Medicine (IF 7.9), in a 2024 study investigating how downregulated SPESP1-driven fibroblast senescence impairs wound healing in aged mice. The research falls within the fields of aging biology, fibroblast senescence, and wound repair. The antibody was applied in Western blot and immunofluorescence for both human and mouse samples.

SpeciesApplicationTitle
human,mouseWB,IF

Clin Transl Med

Downregulated SPESP1-driven fibroblast senescence decreases wound healing in aged mice

Authors - Yun Zhong
  • KD Validated
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