SPHK1 Recombinant monoclonal antibody

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• SPHK1 Recombinant monoclonal antibody (82609-1-RR)

SPHK1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 82609-1-RR Copied!

Clone No. 2K12

Publications (7)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Acetyltransferase SPHK1, EC:2.3.1.-, EC:2.7.1.91, SK1, Sphingosine kinase 1

• 

  WB analysis using 82609-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82609-1-RR (SPHK1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 82609-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SPHK1 antibody (82609-1-RR, Clone: 2K12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 82609-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82609-1-RR against Human SPHK1 were performed. The affinity constant is below 1 pM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells, MCF-7 cells, NIH/3T3 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82609-1-RR targets SPHK1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1051

  Product name: Recombinant human SPHK1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 85-384 aa of BC008040

  Sequence: HEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLLLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL

• Full Name: sphingosine kinase 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC008040
• Gene Symbol: SPHK1
• Gene ID (NCBI): 8877
• RRID: AB_3086491
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NYA1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPHK(Sphingosine kinase 1) is also named as SPHK, SPK.It catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions and is precariously perched at the balance point between progrowth and pro-death signalling in the cell.Endogenous SPHK1 purified from placenta has identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties(PMID:10947957).It has 2 isoforms produced by alternative splicing and can form the oligomeric structure(PMID:22335554).

Publications

What published studies show

The SPHK1 Recombinant monoclonal antibody (2K12), catalog number 82609-1-RR, has 7 citations. These appear in journals including Sci China Life Sci, Phytother Res, Int Immunopharmacol, Biol Direct, BMC Cancer, J Ethnopharmacol, and Mechanobiol Med. The associated research fields span sphingolipid metabolism, tumor immunity, atherosclerosis, renal lipidomics, AML venetoclax resistance, vascular cognitive impairment, and osteogenesis. Applications cited include WB and IF in human and mouse samples.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Sci China Life Sci; SPHK1 deficiency promotes intestinal homeostasis by ameliorating ER stress-induced gastrointestinal injury during murine graft-versus-host disease.; Authors - Di Wu
mouse	WB	Phytother Res; Bioactive Sphingolipids of Cordyceps sinensis Modulate Tumor Immunity Through the SphK1-Mediated Cer/S1P Axis.; Authors - Qi-Xuan Kuang
mouse	WB,IF	Int Immunopharmacol; Isoginkgetin stabilizes atherosclerotic plaques by targeting NR4A2 to modulate the PPAR signaling pathway and reprogram ceramide metabolism, thereby inhibiting vascular smooth muscle cell phenotypic transformation.; Authors - Dongsheng Wei
mouse	WB,IF	Biol Direct; Dapagliflozin induces renal lipidomic remodeling and systemic metabolic improvement.; Authors - Anni Li
human	WB	BMC Cancer; ASAH1-mediated sphingolipid metabolic reprogramming in venetoclax resistance of AML: beyond the monocytic phenotypes.; Authors - Lei Zhao
		J Ethnopharmacol; Jiawei Dingzhi Wan ameliorates chronic cerebral hypoperfusion-induced vascular cognitive impairment by modulating ACER2 and S1P-mediated microvascular angiogenesis and aortic vasoconstriction.; Authors - Cong Fang