SPOCD1 Polyclonal antibody

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  WB analysis of SH-SY5Y using 22243-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22243-1-AP (SPOCD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 22243-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 22243-1-AP (SPOCD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22243-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22243-1-AP (SPOCD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

SPOCD1 Polyclonal Antibody for WB, ELISA

Cat No. 22243-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Synonyms

RP11 84A19.1, SPOC domain containing 1, SPOCD1

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HEK-293 cells, human testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

22243-1-AP targets SPOCD1 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17734

  Product name: Recombinant human SPOCD1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 854-1204 aa of BC114430

  Sequence: PAAPTKALPCLPPWEGVLDMFSIKRFRARAQLVSGHSCRLVQALPTVIRSAGCIPSNIVWDLLASICPAKAKDVCVVRLCPHGARDTQNCRLLYSYLNDRQRHGLASVEHMGMVLLPLPAFQPLPTRLRPLGGPGLEVTHSSLLLAVLLPKEGLPDTAGSSPWLGKVQKMVSFNSKVEKRYYQPDDRRPNVPLKGTPPPGGAWQQSQGRGSIAPRGISAWQRPPRGRGRLWPEPENWQHPGRGQWPPEPGLRQSQHPYSVAPAGHGFGRGQHFHRDSCPHQALLRHLESLATMSHQLQALLCPQTKSSIPRPLQRLSSALAAPEPPGPARDSSLGPTDEAGSECPFPRKA

• Full Name: SPOC domain containing 1
• Calculated Molecular Weight: 1203 aa, 129 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC114430
• Gene Symbol: SPOCD1
• Gene ID (NCBI): 90853
• RRID: AB_2879045
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q6ZMY3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The PIWI protein MIWI2 (PIWIL4; 610315) and its associated PIWI-interacting RNAs (piRNAs) direct DNA methylation of transposable elements in the male germline. SPOCD1 is an essential nuclear effector of MIWI2- and piRNA-directed DNA methylation (PubMed: 32674113).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Oncotarget; Bromodomain protein BRD4 inhibitor JQ1 regulates potential prognostic molecules in advanced renal cell carcinoma.; Authors - Takashi Sakaguchi
human	WB,IF	Cell Death Discov; IFNγ regulates ferroptosis in KFs by inhibiting the expression of SPOCD1 through DNMT3A; Authors - Xiuxia Wang