SPOCD1 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• SPOCD1 Polyclonal antibody (22243-1-AP)

SPOCD1 Polyclonal Antibody for WB, ELISA

Cat No. 22243-1-AP Copied!

Publications (3)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Synonyms

RP11 84A19.1, SPOC domain containing 1, SPOCD1

• 

  WB analysis of SH-SY5Y using 22243-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22243-1-AP (SPOCD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human testis using 22243-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 22243-1-AP (SPOCD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 22243-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22243-1-AP (SPOCD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for SPOCD1 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: SH-SY5Y cells, HEK-293 cells, human testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22243-1-AP targets SPOCD1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17734

  Product name: Recombinant human SPOCD1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 854-1204 aa of BC114430

  Sequence: PAAPTKALPCLPPWEGVLDMFSIKRFRARAQLVSGHSCRLVQALPTVIRSAGCIPSNIVWDLLASICPAKAKDVCVVRLCPHGARDTQNCRLLYSYLNDRQRHGLASVEHMGMVLLPLPAFQPLPTRLRPLGGPGLEVTHSSLLLAVLLPKEGLPDTAGSSPWLGKVQKMVSFNSKVEKRYYQPDDRRPNVPLKGTPPPGGAWQQSQGRGSIAPRGISAWQRPPRGRGRLWPEPENWQHPGRGQWPPEPGLRQSQHPYSVAPAGHGFGRGQHFHRDSCPHQALLRHLESLATMSHQLQALLCPQTKSSIPRPLQRLSSALAAPEPPGPARDSSLGPTDEAGSECPFPRKA

• Full Name: SPOC domain containing 1
• Calculated Molecular Weight: 1203 aa, 129 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC114430
• Gene Symbol: SPOCD1
• Gene ID (NCBI): 90853
• RRID: AB_2879045
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q6ZMY3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The PIWI protein MIWI2 (PIWIL4; 610315) and its associated PIWI-interacting RNAs (piRNAs) direct DNA methylation of transposable elements in the male germline. SPOCD1 is an essential nuclear effector of MIWI2- and piRNA-directed DNA methylation (PubMed: 32674113).

Publications

What published studies show

The SPOCD1 Polyclonal antibody (Cat# 22243-1-AP) has 3 citations published in Cell Death Discovery, Oncotarget, and World Journal of Surgical Oncology. The associated research fields include ferroptosis regulation in kidney fibroblasts, prognostic biomarkers in advanced renal cell carcinoma, and SPOCD1 expression implications for prognosis and immunotherapy in clear cell renal cell carcinoma. All citations involve human samples using WB, IF, or IHC applications.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Cell Death Discov; IFNγ regulates ferroptosis in KFs by inhibiting the expression of SPOCD1 through DNMT3A; Authors - Xiuxia Wang
human	WB	Oncotarget; Bromodomain protein BRD4 inhibitor JQ1 regulates potential prognostic molecules in advanced renal cell carcinoma.; Authors - Takashi Sakaguchi
human	WB,IHC	World J Surg Oncol; SPOCD1 expression in clear cell renal cell carcinoma: its implications for the prognosis and immunotherapy.; Authors - Wu Xu