SPOCD1 Polyclonal antibody, PBS Only

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• SPOCD1 Polyclonal antibody, PBS Only (22243-1-PBS)

• 

  WB analysis of SH-SY5Y using 22243-1-AP (same clone as 22243-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22243-1-AP (SPOCD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22243-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 22243-1-AP (same clone as 22243-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 22243-1-AP (SPOCD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22243-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 22243-1-AP (same clone as 22243-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22243-1-AP (SPOCD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22243-1-PBS in a different storage buffer formulation.

SPOCD1 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 22243-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

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Product Information

22243-1-PBS targets SPOCD1 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17734

  Product name: Recombinant human SPOCD1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 854-1204 aa of BC114430

  Sequence: PAAPTKALPCLPPWEGVLDMFSIKRFRARAQLVSGHSCRLVQALPTVIRSAGCIPSNIVWDLLASICPAKAKDVCVVRLCPHGARDTQNCRLLYSYLNDRQRHGLASVEHMGMVLLPLPAFQPLPTRLRPLGGPGLEVTHSSLLLAVLLPKEGLPDTAGSSPWLGKVQKMVSFNSKVEKRYYQPDDRRPNVPLKGTPPPGGAWQQSQGRGSIAPRGISAWQRPPRGRGRLWPEPENWQHPGRGQWPPEPGLRQSQHPYSVAPAGHGFGRGQHFHRDSCPHQALLRHLESLATMSHQLQALLCPQTKSSIPRPLQRLSSALAAPEPPGPARDSSLGPTDEAGSECPFPRKA

• Full Name: SPOC domain containing 1
• Calculated Molecular Weight: 1203 aa, 129 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC114430
• Gene Symbol: SPOCD1
• Gene ID (NCBI): 90853
• RRID: AB_2879045
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q6ZMY3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The PIWI protein MIWI2 (PIWIL4; 610315) and its associated PIWI-interacting RNAs (piRNAs) direct DNA methylation of transposable elements in the male germline. SPOCD1 is an essential nuclear effector of MIWI2- and piRNA-directed DNA methylation (PubMed: 32674113).