SPP2 Polyclonal antibody

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  WB analysis using 30922-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30922-1-AP (SPP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

SPP2 Polyclonal Antibody for WB, ELISA

Cat No. 30922-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

SPP24, Spp 24, Secreted phosphoprotein 24, Secreted phosphoprotein 2

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Tested Applications

• Positive WB detected in: human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30922-1-AP targets SPP2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPP2 fusion protein Ag29299
• Full Name: secreted phosphoprotein 2, 24kDa
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: NM_006944
• Gene Symbol: SPP2
• Gene ID (NCBI): 6694
• RRID: AB_3669785
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13103
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPP2 (Secreted phosphoprotein 24), also known as SPP24. SPP2 is a secreted phosphoprotein, which was originally isolated and purified from bovine bone density. It is widely distributed in the body, mainly produced in the liver and transported to other tissues. SPP2 is expressed in many tissues, especially in the liver. Studies have shown that the expression level of SPP2 is up-regulated in liver cirrhosis. In addition, the expression of SPP2 is significantly related to many genes, including APOA1, SLC2A2 and TTR (PMID: 38448371). SPP2 has many biological functions, including: it can inhibit the activity of cysteine protease, participate in bone formation, inhibit calcification, and regulate BMP/TGF-β signaling pathway. SPP2 may be a tumor suppressor, which inhibits the development of HCC by inhibiting BMP/TGF-β signaling pathway. The molecular weight of SPP2 is 24 kDa.