SPTAN1 Recombinant antibody

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  WB analysis using 84761-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84761-2-RR (SPTAN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 84761-2-RR

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 84761-2-RR (SPTAN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 84761-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84761-2-RR (SPTAN1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 84761-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84761-2-RR (SPTAN1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 84761-2-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84761-2-RR (SPTAN1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 84761-2-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84761-2-RR (SPTAN1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84761-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84761-2-RR against Human SPTAN1 were performed. The affinity constant is 2.59 nM.

SPTAN1 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 84761-2-RR

Clone No.242104B7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

242104B7, Alpha II spectrin, NEAS, SPTA2

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Tested Applications

• Positive WB detected in: 3T3-L1 cells, mouse brain tissue, HeLa cells, Jurkat cells, MDA-MB-231 cells, rat brain tissue
• Positive IHC detected in: mouse brain tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84761-2-RR targets SPTAN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SPTAN1 fusion protein Ag36472
• Full Name: spectrin, alpha, non-erythrocytic 1 (alpha-fodrin)
• Calculated Molecular Weight: 285kDa，2472aa
• Observed Molecular Weight: 285 kDa,150 kDa
• GenBank Accession Number: NM_003127.3
• Gene Symbol: SPTAN1
• Gene ID (NCBI): 6709
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13813
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPTAN1 (Nonerythroid spectrin αII), also termed α-Fodrin, is a cytoskeletal protein that belongs to the family of spectrins. Spectrins includes several structural proteins (α- and β-spectrin, α-actinin, dystrophin, and utrophin) that build and stabilize the cytoskeleton by forming a hexagonal mesh under the plasma membrane and ensuring stability and organization of organelles in the cell. SPTAN1 encodes for a 2,472 amino acid protein with a predicted molecular weight of 284 kDa and can be cleaved by calpain leading to a 150 kDa fragment (PMID: 31186638).