SR140 Polyclonal antibody

SR140 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 21399-1-AP

Synonyms

KIAA0332, SR140, U2 associated protein SR140, U2 associated SR140 protein, U2SURP



Tested Applications

Positive WB detected inmouse heart tissue, HeLa cells, human brain tissue, L02 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells, Hela cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21399-1-AP is SR140 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SR140 fusion protein Ag16093
Full Name U2-associated SR140 protein
Calculated molecular weight 1029 aa, 118 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC111692
Gene symbol SR140
Gene ID (NCBI) 23350
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SR140 antibody 21399-1-APDownload protocol
IHC protocol for SR140 antibody 21399-1-APDownload protocol
IF protocol for SR140 antibody 21399-1-APDownload protocol
Standard Protocols
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