SR140 Polyclonal antibody, PBS Only

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• SR140 Polyclonal antibody, PBS Only (21399-1-PBS)

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  WB analysis of mouse heart using 21399-1-AP (same clone as 21399-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21399-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 21399-1-AP (same clone as 21399-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21399-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 21399-1-AP (same clone as 21399-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21399-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 21399-1-AP (same clone as 21399-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21399-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 21399-1-AP (same clone as 21399-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21399-1-AP (SR140 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 21399-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 21399-1-AP (same clone as 21399-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21399-1-AP (SR140 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 21399-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 21399-1-AP (same clone as 21399-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SR140 antibody (21399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 21399-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 21399-1-AP (same clone as 21399-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SR140 antibody (21399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 21399-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 21399-1-AP (same clone as 21399-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SR140 antibody (21399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 21399-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 21399-1-AP (same clone as 21399-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using SR140 antibody 21399-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 21399-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 21399-1-AP (same clone as 21399-1-PBS)

  Immunofluorescent analysis of Hela cells, using SR140 antibody 21399-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 21399-1-PBS in a different storage buffer formulation.

SR140 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 21399-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

21399-1-PBS targets SR140 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16093

  Product name: Recombinant human SR140 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 685-1029 aa of BC111692

  Sequence: KETEDVPDDLDGAPIEEELDGAPLEDVDGIPIDATPIDDLDGVPIKSLDDDLDGVPLDATEDSKKNEPIFKVAPSKWEAVDESELEAQAVTTSKWELFDQHEESEEEENQNQEEESEDEEDTQSSKSEEHHLYSNPIKEEMTESKFSKYSEMSEEKRAKLREIELKVMKFQDELESGKRPKKPGQSFQEQVEHYRDKLLQREKEKELERERERDKKDKEKLESRSKDKKEKDECTPTRKERKRRHSTSPSPSRSSSGRRVKSPSPKSERSERSERSHKESSRSRSSHKDSPRDVSKKAKRSPSGSRTPKRSRRSRSRSPKKSGKKSRSQSRSPHRSHKKSKKNKH

• Full Name: U2-associated SR140 protein
• Calculated Molecular Weight: 1029 aa, 118 kDa
• Observed Molecular Weight: 120-140 kDa
• GenBank Accession Number: BC111692
• Gene Symbol: SR140
• Gene ID (NCBI): 23350
• RRID: AB_10863661
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15042
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.