SREBF1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SREBF1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B6G5

Cat no : 66875-1-PBS

Synonyms

bHLHd1, SREBF1, SREBP 1, SREBP 1c, SREBP1

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Validation Data Gallery

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  WB analysis using 66875-1-Ig (same clone as 66875-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66875-1-Ig (SREBF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66875-1-Ig (same clone as 66875-1-PBS)

  WB result of SREBF1 antibody (66875-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SREBF1 transfected HeLa cells. This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66875-1-Ig (same clone as 66875-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66875-1-Ig (SREBF1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66875-1-Ig (same clone as 66875-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66875-1-Ig (SREBF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66875-1-Ig (same clone as 66875-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66875-1-Ig (SREBF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66875-1-Ig (same clone as 66875-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66875-1-Ig (SREBF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66875-1-Ig (same clone as 66875-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SREBF1 antibody (66875-1-Ig, Clone: 1B6G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). CL594-Phalloidin stains the F-actin (red). DAPI (blue). This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66875-1-Ig (same clone as 66875-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66875-1-Ig (SREBF1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66875-1-PBS targets SREBF1 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SREBF1 fusion protein Ag5484
• Full Name: sterol regulatory element binding transcription factor 1
• Calculated Molecular Weight: 1177 aa, 125 kDa
• Observed Molecular Weight: 125 kDa
• GenBank Accession Number: BC063281
• Gene Symbol: SREBF1
• Gene ID (NCBI): 6720
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

SREBF1, also named as BHLHD1 and SREBP1, contains one basic helix-loop-helix (bHLH) domain and belongs to the SREBP family. It is a transcriptional activator required for lipid homeostasis. The SREBPs are synthesized as precursors anchored to endoplasmic reticulum (ER) membranes and complexed with SCAP. When the cellular cholesterol level is low, SREBP-SCAP complexes move to the Golgi apparatus, where SREBPs undergo a two-step proteolytic processing, leading to the release of the mature form, an N-terminal fragment, i.e, basic helix-loop-helix leucine zipper transcription factor. These factors enter the nucleus where they bind to sterol regulatory elements (SRE) in the promoter regions of a number of genes whose products mediate the synthesis of cholesterol and fatty acids. [PMID: 21698267]. This antibody can recognize the 125kd precursor form and the 68kd mature form of human SREBF1.