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SREBF1 Monoclonal antibody
SREBF1 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Human and More (2)
WB, IP, IHC, IF, ELISA
Cat no : 66875-1-Ig
Validation Data Gallery
|Positive WB detected in||A549 cells, Jurkat cells, MCF-7 cells, HeLa cells, L02 cells, HT-29 cells|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
|WB||See 23 publications below|
|IHC||See 2 publications below|
|IF||See 3 publications below|
|IP||See 1 publications below|
66875-1-Ig targets SREBF1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human samples.
|Cited Reactivity||human, bovine|
|Host / Isotype||Mouse / IgG1|
|Immunogen||SREBF1 fusion protein Ag5484|
|Full Name||sterol regulatory element binding transcription factor 1|
|Calculated molecular weight||1177 aa, 125 kDa|
|Observed molecular weight||125 kDa|
|GenBank accession number||BC063281|
|Gene ID (NCBI)||6720|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
SREBF1, also named as BHLHD1 and SREBP1, contains one basic helix-loop-helix (bHLH) domain and belongs to the SREBP family. It is a transcriptional activator required for lipid homeostasis. The SREBPs are synthesized as precursors anchored to endoplasmic reticulum (ER) membranes and complexed with SCAP. When the cellular cholesterol level is low, SREBP-SCAP complexes move to the Golgi apparatus, where SREBPs undergo a two-step proteolytic processing, leading to the release of the mature form, an N-terminal fragment, i.e, basic helix-loop-helix leucine zipper transcription factor. These factors enter the nucleus where they bind to sterol regulatory elements (SRE) in the promoter regions of a number of genes whose products mediate the synthesis of cholesterol and fatty acids. [PMID: 21698267]. This antibody can recognize the 125kd precursor form and the 68kd mature form of human SREBF1.
|Product Specific Protocols|
|WB protocol for SREBF1 antibody 66875-1-Ig||Download protocol|
|IHC protocol for SREBF1 antibody 66875-1-Ig||Download protocol|
|IF protocol for SREBF1 antibody 66875-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Iram (Verified Customer) (08-14-2020)
we saw very clean staining of SREBF1 in our tissue sections.
Miray (Verified Customer) (11-25-2019)
it works well for the liver lysate isolated from mice liver.