SREBF1 Monoclonal antibody

• Featured Product
• KD/KO Validated

SREBF1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

26

CloneNo.

1B6G5

Reviews (2)

Cat no : 66875-1-Ig

Synonyms

bHLHd1, SREBF1, SREBP 1, SREBP 1c, SREBP1

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Validation Data Gallery

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  WB analysis using 66875-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66875-1-Ig (SREBF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66875-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66875-1-Ig (SREBF1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66875-1-Ig

  WB result of SREBF1 antibody (66875-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SREBF1 transfected HeLa cells.

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  WB analysis using 66875-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66875-1-Ig (SREBF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 66875-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66875-1-Ig (SREBF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 66875-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66875-1-Ig (SREBF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66875-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SREBF1 antibody (66875-1-Ig, Clone: 1B6G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). CL594-Phalloidin stains the F-actin (red). DAPI (blue).

• 

  IF Staining of HepG2 using 66875-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66875-1-Ig (SREBF1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, Jurkat cells, MCF-7 cells, HeLa cells, L02 cells, HT-29 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 23 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

66875-1-Ig targets SREBF1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, bovine
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SREBF1 fusion protein Ag5484
• Full Name: sterol regulatory element binding transcription factor 1
• Calculated molecular weight: 1177 aa, 125 kDa
• Observed molecular weight: 125 kDa
• GenBank accession number: BC063281
• Gene symbol: SREBF1
• Gene ID (NCBI): 6720
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SREBF1, also named as BHLHD1 and SREBP1, contains one basic helix-loop-helix (bHLH) domain and belongs to the SREBP family. It is a transcriptional activator required for lipid homeostasis. The SREBPs are synthesized as precursors anchored to endoplasmic reticulum (ER) membranes and complexed with SCAP. When the cellular cholesterol level is low, SREBP-SCAP complexes move to the Golgi apparatus, where SREBPs undergo a two-step proteolytic processing, leading to the release of the mature form, an N-terminal fragment, i.e, basic helix-loop-helix leucine zipper transcription factor. These factors enter the nucleus where they bind to sterol regulatory elements (SRE) in the promoter regions of a number of genes whose products mediate the synthesis of cholesterol and fatty acids. [PMID: 21698267]. This antibody can recognize the 125kd precursor form and the 68kd mature form of human SREBF1.

Publications

Species	Application	Title
human	WB	Cell Metab; TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.; Authors - Dong Huang
		J Hepatol; OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.; Authors - Weiqi Dai
human	WB	Nat Commun; Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis in models of non-alcoholic fatty liver disease; Authors - Evangelia Lekka
human,	WB	J Hazard Mater; In vitro effects of Triclocarban on adipogenesis in murine preadipocyte and human hepatocyte.; Authors - Manyuan Dong
human	WB	Anal Chem; CD36 as a Molecular Target of Functional DNA Aptamer NAFLD01 Selected against NAFLD Cells.; Authors - Ying Pu
mouse	WB,IHC	Cell Mol Life Sci; Inhibition of ALG3 stimulates cancer cell immunogenic ferroptosis to potentiate immunotherapy.; Authors - Pei Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iram (Verified Customer) (08-14-2020)

we saw very clean staining of SREBF1 in our tissue sections.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000 for western and 1:100 for IHC
• Cell Tissue Type: Human colon cancer cells

FH

Miray (Verified Customer) (11-25-2019)

it works well for the liver lysate isolated from mice liver.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Liver

SREBF1 Antibody Western Blot, validation (1:1000 dilution) in Liver (Cat no:66875-1-Ig)