SREBF2 Polyclonal antibody

SREBF2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, chIP,ELISA

Conjugate

Unconjugated

Cat no : 14508-1-AP

Synonyms

bHLHd2, SREBF2, SREBP 2, SREBP2



Tested Applications

Positive WB detected inJurkat cells, mouse kidney tissue, HepG2 cells, human kidney tissue, HEK-293 cells, rat kidney tissue, HeLa cells, rat liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue, human kidney tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14508-1-AP targets SREBF2 in WB, IP, IHC, chIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity chicken, Hamster, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SREBF2 fusion protein Ag5967
Full Name sterol regulatory element binding transcription factor 2
Calculated molecular weight 124 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC056158
Gene symbol SREBF2
Gene ID (NCBI) 6721
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SREBF2,also named as BHLHD2, is a 1141 amino acid protein, which contains 1 bHLH domain and belongs to the SREBP family. SREBF2 localizes in the endoplasmic reticulum membrane and is ubiquitously expressed in adult and fetal tissues. SREBF2 as a transcriptional activator is required for lipid homeostasis. SREBF2 expression is dynamically regulated in response to nutritional and hormonal cues.

Protocols

Product Specific Protocols
WB protocol for SREBF2 antibody 14508-1-APDownload protocol
IHC protocol for SREBF2 antibody 14508-1-APDownload protocol
IP protocol for SREBF2 antibody 14508-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

SREBP-dependent lipidomic reprogramming as a broad-spectrum antiviral target.

Authors - Shuofeng Yuan
  • KD Validated
humanWB

Int J Mol Med

Hydrogen sulfide inhibits PCSK9 expression through the PI3K/Akt‑SREBP‑2 signaling pathway to influence lipid metabolism in HepG2 cells.

Authors - Jun Xiao
mouseWB

J Agric Food Chem

Lonicera caerulea Berry Polyphenols Activate SIRT1, Enhancing Inhibition of Raw264.7 Macrophage Foam Cell Formation and Promoting Cholesterol Efflux.

Authors - Suwen Liu
ratWB

Int J Clin Exp Pathol

Ethyl acetate extract of sappanwood alleviates experimental atherosclerosis in rats through changes in FGF21 and SREBP-2 expression.

Authors - Quan Li
humanWB

Exp Ther Med

Apigenin reduces the excessive accumulation of lipids induced by palmitic acid via the AMPK signaling pathway in HepG2 cells.

Authors - Jing Lu
hamsterWB

J Food Drug Anal

Red algae (Gelidium amansii) hot-water extract ameliorates lipid metabolism in hamsters fed a high-fat diet.

Authors - Tsung-Han Yang