|Positive WB detected in||Jurkat cells, mouse kidney tissue, HepG2 cells, human kidney tissue, HEK-293 cells, rat kidney tissue, HeLa cells, rat liver tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||mouse brain tissue, human kidney tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14508-1-AP targets SREBF2 in WB, IP, IHC, chIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||chicken, Hamster, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SREBF2 fusion protein Ag5967|
|Full Name||sterol regulatory element binding transcription factor 2|
|Calculated molecular weight||124 kDa|
|Observed molecular weight||68 kDa|
|GenBank accession number||BC056158|
|Gene ID (NCBI)||6721|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SREBF2,also named as BHLHD2, is a 1141 amino acid protein, which contains 1 bHLH domain and belongs to the SREBP family. SREBF2 localizes in the endoplasmic reticulum membrane and is ubiquitously expressed in adult and fetal tissues. SREBF2 as a transcriptional activator is required for lipid homeostasis. SREBF2 expression is dynamically regulated in response to nutritional and hormonal cues.
SREBP-dependent lipidomic reprogramming as a broad-spectrum antiviral target.
Int J Mol Med
Hydrogen sulfide inhibits PCSK9 expression through the PI3K/Akt‑SREBP‑2 signaling pathway to influence lipid metabolism in HepG2 cells.
J Agric Food Chem
Lonicera caerulea Berry Polyphenols Activate SIRT1, Enhancing Inhibition of Raw264.7 Macrophage Foam Cell Formation and Promoting Cholesterol Efflux.
Int J Clin Exp Pathol
Ethyl acetate extract of sappanwood alleviates experimental atherosclerosis in rats through changes in FGF21 and SREBP-2 expression.
Exp Ther Med
Apigenin reduces the excessive accumulation of lipids induced by palmitic acid via the AMPK signaling pathway in HepG2 cells.
J Food Drug Anal
Red algae (Gelidium amansii) hot-water extract ameliorates lipid metabolism in hamsters fed a high-fat diet.