SRGAP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SRGAP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 22519-1-AP

Synonyms

ARHGAP34, FNBP2, Formin binding protein 2, KIAA0456, SRGAP2, srGAP3

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Validation Data Gallery

• 

  WB analysis of mouse kidney using 22519-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22519-1-AP (SRGAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22519-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22519-1-AP (SRGAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 22519-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22519-1-AP (SRGAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 22519-1-AP

  IP result of anti-SRGAP2 (IP:22519-1-AP, 4ug; Detection:22519-1-AP 1:1000) with HEK-293 cells lysate 3000ug.

• 

  IHC staining of human kidney using 22519-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 22519-1-AP (SRGAP2 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

22519-1-AP targets SRGAP2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SRGAP2 fusion protein Ag18278
• Full Name: SLIT-ROBO Rho GTPase activating protein 2
• Calculated Molecular Weight: 1071 aa, 121 kDa
• Observed Molecular Weight: 121 kDa
• GenBank Accession Number: BC132874
• Gene Symbol: SRGAP2
• Gene ID (NCBI): 23380
• RRID: AB_2879114
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC,IF	Theranostics; Stiff matrix induced srGAP2 tension gradients control migration direction in triple-negative breast cancer; Authors - Chen Li ; KD Validated
human	WB	Nat Commun; Haploinsufficiency in the ANKS1B gene encoding AIDA-1 leads to a neurodevelopmental syndrome.; Authors - Abigail U Carbonell
mouse	WB	Cell Rep; Huntingtin coordinates dendritic spine morphology and function through cofilin-mediated control of the actin cytoskeleton; Authors - Doris Wennagel
mouse	WB,IHC	Biomed Pharmacother; CDDO-Me ameliorates podocyte injury through anti-oxidative stress and regulation of actin cytoskeleton in adriamycin nephropathy; Authors - Cheng Xu