Spermidine synthase Polyclonal antibody

Spermidine synthase Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

12

Reviews (1)

Cat no : 19858-1-AP

Synonyms

SRM, SRML1, SPS1, SPDSY, PAPT

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Validation Data Gallery

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  WB analysis using 19858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19858-1-AP (Spermidine synthase antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 19858-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19858-1-AP (Spermidine synthase antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 19858-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 19858-1-AP (Spermidine synthase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 19858-1-AP

  IP result of anti-Spermidine synthase (IP:19858-1-AP, 4ug; Detection:19858-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human kidney using 19858-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19858-1-AP (Spermidine synthase antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 19858-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19858-1-AP (Spermidine synthase antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 19858-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19858-1-AP (Spermidine synthase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 19858-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Spermidine synthase antibody (19858-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, LNCaP cells, HepG2 cells, PC-3 cells, mouse liver tissue, rat liver tissue, RAW 264.7 cells, mouse brain tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human kidney tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

19858-1-AP targets Spermidine synthase in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Spermidine synthase fusion protein Ag13936
• Full Name: spermidine synthase
• Calculated Molecular Weight: 302 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC000309
• Gene Symbol: Spermidine synthase
• Gene ID (NCBI): 6723
• RRID: AB_10665555
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Spermidine synthase is an enzyme that catalyzes the transfer of the propylamine group from S-adenosylmethioninamine to putrescine in the biosynthesis of spermidine. The polyamines putrescine, spermine, and spermidine are ubiquitous polycationic mediators of cell growth and differentiation. Spermidine synthases catalyze the production of the linear triamine, spermidine, from putrescine. They utilize decarboxylated S-adenosylmethionine (dc-SAM), a universal cofactor of aminopropyltransferases, as a donor of the aminopropyl moiety (PMID:31134111).

Publications

Species	Application	Title
mouse	WB	J Clin Invest; Aberrant AZIN2 and polyamine metabolism precipitates tau neuropathology.; Authors - Leslie A Sandusky-Beltran
human	WB,IF	Nat Commun; Macrophage ABHD5 promotes colorectal cancer growth by suppressing spermidine production by SRM.; Authors - Hongming Miao
human	WB	Cancer Sci; The miR-1908/SRM regulatory axis contributes to extracellular vesicle secretion in prostate cancer.; Authors - Fumihiko Urabe
mouse	WB	J Proteomics; Proteomic analysis revealed different responses to hypergravity of soleus and extensor digitorum longus muscles in mice.; Authors - Takashi Ohira
mouse	WB	Am J Physiol Cell Physiol; The Regulation of Polyamine Pathway Proteins in Models of Skeletal Muscle Hypertrophy and Atrophy - a potential role for mTORC1.; Authors - Michael Tabbaa
	IHC	Biol Reprod; Progesterone and interferon tau regulate expression of polyamine enzymes during the ovine peri-implantation period.; Authors - Katherine M Halloran

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (08-15-2024)

Good antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse liver

Spermidine synthase Antibody Western Blot validation (1:1000 dilution) in Mouse liver (Cat no:19858-1-AP)