SRP68 Polyclonal antibody, PBS Only

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• SRP68 Polyclonal antibody, PBS Only (11585-1-PBS)

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  WB analysis of mouse brain using 11585-1-AP (same clone as 11585-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11585-1-AP (SRP68 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11585-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11585-1-AP (same clone as 11585-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11585-1-AP (SRP68 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11585-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 11585-1-AP (same clone as 11585-1-PBS)

  IP result of anti-SRP68 (IP:11585-1-AP, 4ug; Detection:11585-1-AP 1:400) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 11585-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 11585-1-AP (same clone as 11585-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11585-1-AP (SRP68 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11585-1-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 11585-1-AP (same clone as 11585-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11585-1-AP (SRP68 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11585-1-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 11585-1-AP (same clone as 11585-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11585-1-AP (SRP68 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11585-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 11585-1-AP (same clone as 11585-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11585-1-AP (SRP68 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11585-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 11585-1-AP (same clone as 11585-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SRP68 antibody (11585-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11585-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SRP68 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 11585-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

SRP 68, Signal recognition particle subunit SRP68

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Product Information

11585-1-PBS targets SRP68 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2177

  Product name: Recombinant human SRP68 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 262-596 aa of BC020238

  Sequence: MSEVEWRGRTVPVKIDKVRIFLLGLADNEAAIVQAESEETKERLFESMLSECRDAIQVVREELKPDQKQRDYILEGEPGKVSNLQYLHSYLTYIKLSTAIKRNENMAKGLQRALLQQQPEDDSKRSPRPQDLIRLYDIILQNLVELLQLPGLEEDKAFQKEIGLKTLVFKAYRCFFIAQSYVLVKKWSEALVLYDRVLKYANEVNSDAGAFKNSLKDLPDVQELITQVRSEKCSLQAAAILDANDAHQTETSSSQVKDNKPLVERFETFCLDPSLVTKQANLVHFPPGFQPIPCKPLFFDLALNHVAFPPLEDKLEQKTKSGLTGYIKGIFGFRS

• Full Name: signal recognition particle 68kDa
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC020238
• Gene Symbol: SRP68
• Gene ID (NCBI): 6730
• RRID: AB_2286586
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UHB9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of secretory proteins to the rough endoplasmic reticulum (ER) membrane. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function. SRP68 is one component of the ribonucleprotein complex, and forms a heterodimer with SRP72.