SRY Monoclonal antibody

SRY Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2E9A4

Cat no : 67349-1-Ig

Synonyms

Testis-determining factor, Sex-determining region Y protein, sex determining region Y, 2E9A4

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Validation Data Gallery

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  WB analysis of LNCaP using 67349-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67349-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. SRY negative cell line HeLa cell line was served as negative control.

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  WB analysis of DU 145 using 67349-1-Ig

  DU 145 cells were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: LNCaP cells, DU 145 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67349-1-Ig targets SRY in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SRY fusion protein Ag12610
• Full Name: sex determining region Y
• Calculated Molecular Weight: 204 aa, 24 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC074924
• Gene Symbol: SRY
• Gene ID (NCBI): 6736
• RRID: AB_2882607
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SRY (sex-determining region Y protein) is a tran-scriptional activator required for male sex determination in mammals. This protein, also referred to as testis-determining factor (TDF), is an HMG box protein that initiates the formation of testis from undifferentiated gonad. The DNA-binding activity of SRY is required for normal testis formation. This DNA-binding activity is thought to be regulated by PKA, which phosphorylates SRY in vivo. Mutations in SRY have been associated with 46,XY gonadal dysgenesis, in which the gonads fail to develop in XY phenotypic females.

Publications

Species	Application	Title
human	WB	Cell Rep; Recruitment of DNA to tumor-derived microvesicles.; Authors - James W Clancy