SSB Recombinant monoclonal antibody, PBS Only

Name: SSB Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 85944-2-PBS-100UG

SSB Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 85944-2-PBS

Clone No. 250465G10

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

La, La autoantigen, La ribonucleoprotein, LARP3, Lupus La protein

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIF/ICC

WB analysis using 85944-2-RR (same clone as 85944-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 85944-2-RR (SSB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85944-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 85944-2-RR (same clone as 85944-2-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SSB antibody (85944-2-RR, Clone: 250465G10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85944-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for SSB in ?

85944-2-PBS targets SSB in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag2294 Product name: Recombinant human SSB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-408 aa of BC001289 Sequence: MAENGDNEKMAALEAKICHQIEYYFGDFNLPRDKFLKEQIKLDEGWVPLEIMIKFNRLNRLTTDFNVIVEALSKSKAELMEISEDKTKIRRSPSKPLPEVTDEYKNDVKNRSVYIKGFPTDATLDDIKEWLEDKGQVLNIQMRRTLHKAFKGSIFVVFDSIESAKKFVETPGQKYKETDLLILFKDDYFAKKNEERKQNKVEAKLRAKQEQEAKQKLEEDAEMKSLEEKIGCLLKFSGDLDDQTCREDLHILFSNHGEIKWIDFVRGAKEGIILFKEKAKEALGKAKDANNGNLQLRNKEVTWEVLEGEVEKEALKKIIEDQQESLNKWKSKGRRFKGKGKGNKAAQPGSGKGKVQFQGKKTKFASDDEHDEHDENGATGPVKRAREETDKEEPASKQQKTENGAGDQ Predict reactive species
Full Name	Sjogren syndrome antigen B (autoantigen La)
Calculated Molecular Weight	408 aa, 47 kDa
Observed Molecular Weight	46-50 kDa
GenBank Accession Number	BC001289
Gene Symbol	SSB
Gene ID (NCBI)	6741
RRID	AB_3744493
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P05455
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.