CoraLite® Plus 488-conjugated SSB Recombinant monoclonal antibody

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  IF Staining of HeLa using CL488-85944-2

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 SSB antibody (CL488-85944-2, Clone: 250465G10 ) at dilution of 1:200, CL594-phalloidin (red).

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  IF Staining of U-251 using CL488-85944-2

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using CoraLite® Plus 488 SSB antibody (CL488-85944-2, Clone: 250465G10 ) at dilution of 1:200, CL594-phalloidin (red).

SSB Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-85944-2

Clone No.250465G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

La, La autoantigen, La ribonucleoprotein, LARP3, Lupus La protein

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Tested Applications

• Positive IF/ICC detected in: HeLa cells, U-251 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-85944-2 targets SSB in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2294

  Product name: Recombinant human SSB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-408 aa of BC001289

  Sequence: MAENGDNEKMAALEAKICHQIEYYFGDFNLPRDKFLKEQIKLDEGWVPLEIMIKFNRLNRLTTDFNVIVEALSKSKAELMEISEDKTKIRRSPSKPLPEVTDEYKNDVKNRSVYIKGFPTDATLDDIKEWLEDKGQVLNIQMRRTLHKAFKGSIFVVFDSIESAKKFVETPGQKYKETDLLILFKDDYFAKKNEERKQNKVEAKLRAKQEQEAKQKLEEDAEMKSLEEKIGCLLKFSGDLDDQTCREDLHILFSNHGEIKWIDFVRGAKEGIILFKEKAKEALGKAKDANNGNLQLRNKEVTWEVLEGEVEKEALKKIIEDQQESLNKWKSKGRRFKGKGKGNKAAQPGSGKGKVQFQGKKTKFASDDEHDEHDENGATGPVKRAREETDKEEPASKQQKTENGAGDQ

• Full Name: Sjogren syndrome antigen B (autoantigen La)
• Calculated Molecular Weight: 408 aa, 47 kDa
• Observed Molecular Weight: 46-50 kDa
• GenBank Accession Number: BC001289
• Gene Symbol: SSB
• Gene ID (NCBI): 6741
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05455
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.