SSH2 Polyclonal antibody

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• SSH2 Polyclonal antibody (20674-1-AP)

SSH2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20674-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

hSSH 2L, KIAA1725, SSH 2, SSH 2L, SSH like protein 2, SSH2, SSH2L

• 

  WB analysis using 20674-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20674-1-AP (SSH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 20674-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20674-1-AP (SSH2 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 20674-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20674-1-AP (SSH2 Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, K-562 cells, MG-63 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20674-1-AP targets SSH2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: slingshot homolog 2 (Drosophila)
• Calculated Molecular Weight: 158 kDa
• Observed Molecular Weight: 45-51 kDa
• GenBank Accession Number: NM_033389
• Gene Symbol: SSH2
• Gene ID (NCBI): 85464
• RRID: AB_2935449
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q76I76
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The SSH2 polyclonal antibody (Cat# 20674-1-AP) has 1 total citation. It appears in Cell Adhesion & Migration (IF 4.3), published in 2018. The cited study investigated how atypical protein kinase C–activated Cofilin modulates actin dynamics to regulate metastasis in colorectal cancer cells, using the antibody for Western blot in human samples. The research field encompasses cancer metastasis, cytoskeletal regulation, and cell adhesion/migration biology.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Adh Migr; The modulation of actin dynamics via atypical protein kinase-C activated Cofilin regulates metastasis of colorectal cancer cells.; Authors - S M Anisul Islam