Tested Applications
| Positive WB detected in | COS-7 cells, HepG2 cells, human liver tissue, mouse liver tissue, L02 cells |
| Positive IHC detected in | human ovary tumor tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10583-1-AP targets TRAPA/SSR1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, monkey samples.
| Tested Reactivity | human, mouse, monkey |
| Cited Reactivity | human, rat, pig, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0878 Product name: Recombinant human TRAPA, SSR1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-286 aa of BC007710 Sequence: MRLLPRLLLLLLLVFPATVLFRGGPRGLLAVAQDLTEDEETVEDSIIEDEDDEAEVEEDEPTDLVEDKEEEDVSGEPEASPSADTTILFVKGEDFPANNIVKFLVGFTNKGTEDFIVESLDASFRYPQDYQFYIQNFTALPLNTVVPPQRQATFEYSFIPAEPMGGRPFGLVINLNYKDLNGNVFQDAVFNQTVTVIEREDGLDGETIFMYMFLAGLGLLVIVGLHQLLESRKRKRPIQKVEMGTSSQNDVDMSWIPQETLNQINKASPRRLPRKRAQKRSVGSDE Predict reactive species |
| Full Name | signal sequence receptor, alpha |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC007710 |
| Gene Symbol | TRAPA |
| Gene ID (NCBI) | 6745 |
| RRID | AB_2195616 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P43307 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRAPA (translocon-associated protein subunit alpha), also known as SSR1 (signal sequence receptor subunit alpha), is a 34-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 8050590). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRAPA/SSR1 antibody 10583-1-AP | Download protocol |
| IHC protocol for TRAPA/SSR1 antibody 10583-1-AP | Download protocol |
| WB protocol for TRAPA/SSR1 antibody 10583-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRAPA/SSR1 polyclonal antibody (Cat# 10583-1-AP) has 11 citations published in Nature, Cell, Cell Research, Science Advances, Current Biology, Biosensors and Bioelectronics, European Journal of Pharmacology, FASEB Journal, Journal of Virology, JOR Spine, and Experimental Cell Research. Associated research fields include ER biology, organelle distribution, CRISPR screening, NF-κB inflammatory signaling, DNA damage and apoptosis, ER stress, RNA splicing, ER-phagy, ERAD, cancer, viral infection, and intervertebral disc degeneration.
| Species | Application | Title |
|---|---|---|
Cell Positive selection CRISPR screens reveal a druggable pocket in an oligosaccharyltransferase required for inflammatory signaling to NF-κB | ||
Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. | ||
Sci Adv The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress. | ||
Biosens Bioelectron Live imaging of RNA and RNA splicing in mammalian cells via the dcas13a-SunTag-BiFC system. | ||
Reviews
What customers say
Both reviewers rated the antibody 4 out of 5 stars. One user reported it worked well for Western Blot. The other confirmed strong performance across WB, IF, and IP, noting it produced a specific band at the correct molecular weight in A549 and HEK-293 cells at a 1:500 dilution for WB. Overall, users find this anti-SSR1 antibody reliable and specific for its intended applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ann-Kathrin (Verified Customer) (04-13-2026) | The Antibody worked quite well so far.
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Chatchai (Verified Customer) (09-26-2019) | This anti-SSR1 is very good, presents the specific band at the right size of the protein.
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