Tested Applications
| Positive WB detected in | HepG2 cells, L02 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human breast cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10278-1-AP targets TRAPB/SSR2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0304 Product name: Recombinant human TRAPB, SSR2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-175 aa of BC000341 Sequence: MRLLSFVVLALFAVTQAEEGARLLASKSLLNRYAVEGRDLTLQYNIYNVGSSAALDVELSDDSFPPEDFGIVSGMLNVKWDRIAPASNVSHTVVLRPLKAGYFNFTSATITYLAQEDGPVVIGSTSAPGQGGILAQREFDRRFSPHFLDWAAFGVMTLPSIGIPLLLWYSSKRKY Predict reactive species |
| Full Name | signal sequence receptor, beta (translocon-associated protein beta) |
| Calculated Molecular Weight | 20 kDa |
| Observed Molecular Weight | 22-25 kDa |
| GenBank Accession Number | BC000341 |
| Gene Symbol | TRAPB |
| Gene ID (NCBI) | 6746 |
| RRID | AB_2195624 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P43308 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRAPB (translocon-associated protein subunit beta), also known as SSR2 (signal sequence receptor subunit beta), is a 22-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 7789174). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRAPB/SSR2 antibody 10278-1-AP | Download protocol |
| IHC protocol for TRAPB/SSR2 antibody 10278-1-AP | Download protocol |
| WB protocol for TRAPB/SSR2 antibody 10278-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRAPB/SSR2 Polyclonal antibody (Cat# 10278-1-AP) has 13 citations. Journals include Molecular Cell, Science Advances, Journal of Experimental & Clinical Cancer Research, Diabetes, FASEB Journal, Journal of Virology, European Journal of Pharmacology, Journal of Cellular & Molecular Medicine, Biochimica et Biophysica Acta, Journal of Immunology Research, Journal of Cellular Biochemistry, and Current Molecular Medicine. Research fields span endoplasmic reticulum stress, N-glycosylation, proteasomal degradation, hepatocellular carcinoma, colorectal cancer, insulin biosynthesis, ferroptosis, and viral infection.
| Species | Application | Title |
|---|---|---|
Mol Cell RNF126-Mediated Reubiquitination Is Required for Proteasomal Degradation of p97-Extracted Membrane Proteins. | ||
J Exp Clin Cancer Res Long noncoding RNA SNHG14 promotes hepatocellular carcinoma progression by regulating miR-876-5p/SSR2 axis. | ||
Sci Adv Signal recognition particle receptor-β (SR-β) coordinates cotranslational N-glycosylation | ||
Sci Adv The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress. | ||
Diabetes The Role of TRAPγ/SSR3 in Preproinsulin Translocation into the Endoplasmic Reticulum. | ||
Eur J Pharmacol Eeyarestatin I (ESI)-induced ERAD inhibition exhibits anti-cancer activity through multiple mechanisms in human colorectal cancer cells |
