SSR4 Polyclonal antibody

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  WB analysis using 11655-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11655-2-AP (SSR4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 11655-2-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11655-2-AP (SSR4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11655-2-AP

  IP result of anti-SSR4 (IP:11655-2-AP, 4ug; Detection:11655-2-AP 1:300) with HeLa cells lysate 2600 ug.

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  IHC staining of human pancreas cancer using 11655-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11655-2-AP (SSR4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11655-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11655-2-AP (SSR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11655-2-AP

  Immunofluorescent analysis of HeLa cells using 11655-2-AP (SSR4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SSR4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11655-2-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

TRAP-delta, TRAPD, Translocon-associated protein subunit delta, SSR-delta

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Tested Applications

• Positive WB detected in: HeLa cells, human placenta tissue, HepG2 cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 3 publications below

Product Information

11655-2-AP targets SSR4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2250

  Product name: Recombinant human SSR4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-173 aa of BC003371

  Sequence: MAAMASLGALALLLLSSLSRCSAEACLEPQITPSYYTTSDAVISTETVFIVEISLTCKNRVQNMALYADVGGKQFPVTRGQDVGRYQVSWSLDHKSAHAGTYEVRFFDEESYSLLRKAQRNNEDISIIPPLFTVSVDHRGTWNGPWVSTEVLAAAIGLVIYYLAFSAKSHIQA

• Full Name: signal sequence receptor, delta (translocon-associated protein delta)
• Calculated Molecular Weight: 173 aa, 19 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC003371
• Gene Symbol: SSR4
• Gene ID (NCBI): 6748
• RRID: AB_2195738
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P51571
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Signal sequence receptor subunit delta (SSR4, or TRAPD) gene encodes the translocon-associated proteins subunit delta, which is about 19-kDa and is located at endoplasmic reticulum (ER) membrane, the translocon-associated proteins complex functions to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. TRAP-delta can form heterotetramer with other TRAP homolog TRAP-alpha, TRAP-beta and TRAP-gamma.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Sci Adv; The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress.; Authors - Chatchai Phoomak
human	IHC	Expert Rev Mol Diagn; SSR4 as a prognostic biomarker and related with immune infiltration cells in colon adenocarcinoma.; Authors - Weiwei He
human	IHC	Cancer Med; Predicting Outcomes in Esophageal Squamous Cell Carcinoma Using scRNA-Seq and Bulk RNA-Seq: A Model Development and Validation Study; Authors - Jiaqi Zhang
human	WB	J Cancer Prev; Identification of Translocon-associated Protein Delta as An Oncogene in Human Colorectal Cancer Cells; Authors - Darshika Amarakoon
human	IHC	Front Immunol; Unveiling SSR4: a promising biomarker in esophageal squamous cell carcinoma; Authors - Jiaqi Zhang
human	WB	Eur J Pharmacol; Eeyarestatin I (ESI)-induced ERAD inhibition exhibits anti-cancer activity through multiple mechanisms in human colorectal cancer cells; Authors - Jing Peng