SSRP1 Polyclonal antibody

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SSRP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15696-1-AP Copied!

Publications (11)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Chromatin-specific transcription elongation factor 80 kDa subunit, Facilitates chromatin transcription complex 80 kDa subunit, Facilitates chromatin transcription complex subunit SSRP1, FACT, FACT 80 kDa subunit

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  WB analysis of HeLa using 15696-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15696-1-AP (SSRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 15696-1-AP

  WB result of SSRP1 antibody (15696-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SSRP1 transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse testis using 15696-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15696-1-AP (SSRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse testis using 15696-1-AP

  IP result of anti-SSRP1 (IP:15696-1-AP, 4ug; Detection:15696-1-AP 1:500) with mouse testis tissue lysate 2400ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 15696-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15696-1-AP (SSRP1 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 15696-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15696-1-AP (SSRP1 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 15696-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15696-1-AP (SSRP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15696-1-AP targets SSRP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8318

  Product name: Recombinant human SSRP1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 361-709 aa of BC005116

  Sequence: YVHKPPVHIRFDEISFVNFARGTTTTRSFDFEIETKQGTQYTFSSIEREEYGKLFDFVNAKKLNIKNRGLKEGMNPSYDEYADSDEDQHDAYLERMKEEGKIREENANDSSDDSGEETDESFNPGEEEEDVAEEFDSNASASSSSNEGDSDRDEKKRKQLKKAKMAKDRKSRKKPVEVKKGKDPNAPKRPMSAYMLWLNASREKIKSDHPGISITDLSKKAGEIWKGMSKEKKEEWDRKAEDARRDYEKAMKEYEGGRGESSKRDKSKKKKKVKVKMEKKSTPSRGSSSKSSSRQLSESFKSKEFVSSDESSSGENKSKKKRRRSEDSEEEELASTPPSSEDSASGSDE

• Full Name: structure specific recognition protein 1
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC005116
• Gene Symbol: SSRP1
• Gene ID (NCBI): 6749
• RRID: AB_2195756
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q08945
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SSRP1 (Structure-Specific Recognition Protein 1) is one of the key subunits of the chromatin transcription elongation factor FACT (Facilitates Chromatin Transcription) complex. It forms a heterodimer with SUPT16H and is widely involved in DNA replication, repair and transcription processes in eukaryotes.

Publications

What published studies show

The SSRP1 Polyclonal antibody (Cat# 15696-1-AP) has 11 citations published in journals including Molecular Cell, Science Translational Medicine, Advanced Science, Cell Reports, FASEB Journal, Journal of Cachexia Sarcopenia Muscle, and others. Research fields span cancer biology (colorectal, lung, neuroblastoma, hepatocellular carcinoma), chromatin transcription, DNA repair, phase separation, muscle growth, and bone fracture repair. Applications cited include WB, IHC, and CoIP in human and mouse samples.

Automatically generated from the 11 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cell; Aberrant phase separation drives membranous organelle remodeling and tumorigenesis; Authors - Xinyu Wang
human	WB	Sci Transl Med; Therapeutic targeting of the MYC signal by inhibition of histone chaperone FACT in neuroblastoma.; Authors - Daniel R Carter
human	WB,CoIP	Adv Sci (Weinh); Topoisomerase I Inhibition in ETV4-overexpressed Non-Small Cell Lung Cancer Promotes Replication and Transcription Mediated R-Loop Accumulation and DNA Damage; Authors - Jiaxi Zhang
	WB,IHC	J Cachexia Sarcopenia Muscle; A Novel ceRNA Axis LOC121818100/Novel-miR-400/SSRP1 Regulated Muscle Growth and Injury Repair in Sheep; Authors - Fan Yang
human	WB	ACS Appl Mater Interfaces; Injectable Bacteria-Sensitive Hydrogel Promotes Repair of Infected Fractures via Sustained Release of miRNA Antagonist.; Authors - Chenyan Yu ; KD Validated
human	WB	Cell Rep; TRPS1 drives heterochromatic origin refiring and cancer genome evolution.; Authors - Jianguo Yang