SSSCA1 Monoclonal antibody

SSSCA1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D2E8

Cat no : 68423-1-Ig

Synonyms

Autoantigen p27, p27, SSSCA1, ZNRD2



Tested Applications

Positive WB detected inA549 cells, LNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68423-1-Ig targets SSSCA1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SSSCA1 fusion protein Ag16783
Full Name Sjogren syndrome/scleroderma autoantigen 1
Calculated Molecular Weight 199 aa, 21 kDa
Observed Molecular Weight23 kDa
GenBank Accession NumberBC014791
Gene Symbol SSSCA1
Gene ID (NCBI) 10534
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SSSCA1, also named as Autoantigen p27, is centromere-associated protein. It may induce anti-centromere antibodies. SSSCA1 plays a role in mitosis. It's involved in Sjogren syndrome/scleroderma. The calculated MW of SSSCA1 is 21.5 kDa (native form), with modification the MW is about 23-27 kDa.

Protocols

Product Specific Protocols
WB protocol for SSSCA1 antibody 68423-1-IgDownload protocol
IF protocol for SSSCA1 antibody 68423-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols