STAMBPL1 Polyclonal antibody

STAMBPL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 27315-1-AP

Synonyms

ALMalpha, AMSH FP, AMSH like protease, AMSH LP, AMSHLP, KIAA1373, STAM binding protein like 1, STAMBPL1

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Validation Data Gallery

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  WB analysis of human testis using 27315-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 27315-1-AP (STAMBPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27315-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27315-1-AP (STAMBPL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 27315-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27315-1-AP (STAMBPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27315-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27315-1-AP (STAMBPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 27315-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27315-1-AP (STAMBPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 27315-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27315-1-AP (STAMBPL1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 27315-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27315-1-AP (STAMBPL1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 27315-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27315-1-AP (STAMBPL1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 27315-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27315-1-AP (STAMBPL1 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, MCF-7 cells, human testis tissue, Jurkat cells
• Positive IHC detected in: human liver tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

27315-1-AP targets STAMBPL1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STAMBPL1 fusion protein Ag26308
• Full Name: STAM binding protein-like 1
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC010846
• Gene Symbol: STAMBPL1
• Gene ID (NCBI): 57559
• RRID: AB_2880840
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Phytomedicine; A multi-omic analysis reveals that Gamabufotalin exerts anti-hepatocellular carcinoma effects by regulating amino acid metabolism through targeting STAMBPL1; Authors - Piao Zheng
human	WB,IHC	Mol Carcinog; STAMBPL1, transcriptionally regulated by SREBP1, promotes malignant behaviors of hepatocellular carcinoma cells via Wnt/β-catenin signaling pathway; Authors - Junyi Jin
human	WB	Transl Oncol; STAMBPL1 promotes the progression of lung adenocarcinoma by inhibiting DHRS2 expression; Authors - Xiang Yang
human,mouse	WB,IF,CoIP	Cell Rep; A MYC-STAMBPL1-TOE1 positive feedback loop mediates EGFR stability in hepatocellular carcinoma; Authors - Hongli Zhang ; KO Validated