STAR Polyclonal antibody

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• STAR Polyclonal antibody (12225-1-AP)

• KD/KO Validated

STAR Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Cat No. 12225-1-AP Copied!

Publications (98)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (4)

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Synonyms

STARD1, START domain-containing protein 1

• 

  WB analysis using 12225-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12225-1-AP (STAR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 12225-1-AP

  WB result of STAR antibody (12225-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAR transfected K-562 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 12225-1-AP

  Pig adrenal gland lysates were subjected to SDS PAGE followed by western blot with 12225-1-AP (STAR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human adrenal gland using 12225-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 12225-1-AP (STAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 12225-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12225-1-AP (STAR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 12225-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12225-1-AP (STAR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 12225-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12225-1-AP (STAR Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 12225-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12225-1-AP (STAR Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary using 12225-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 12225-1-AP (STAR Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary using 12225-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 12225-1-AP (STAR Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse testis using 12225-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using STAR antibody (12225-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse testis using 12225-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse testis tissue using 12225-1-AP (STAR antibody) at dilution of 1:400 and CoraLite®647-conjugated AffiniPure F(ab, CoraLite®555 CL555-17178 (TNP1 antibody, orange) and CoraLite® Plus 488 CL488-13720 (BOULE antibody, green).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse testis tissue, human adrenal gland tissue, K-562 cells, pig adrenal gland tissue, rat ovary tissue, rat testis tissue
• Positive IHC detected in: mouse testis tissue, human liver cancer tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse testis tissue
• Positive IF-Fro detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12225-1-AP targets STAR in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat, pig, monkey, chicken, bovine, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2862

  Product name: Recombinant human STAR protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-285 aa of BC010550

  Sequence: MLLATFKLCAGSSYRHMRNMKGLRQQAVMAISQELNRRALGGPTPSTWINQVRRRSSLLGSRLEETLYSDQELAYLQQGEEAMQKALGILSNQEGWKKESQQDNGDKVMSKVVPDVGKVFRLEVVVDQPMERLYEELVERMEAMGEWNPNVKEIKVLQKIGKDTFITHELAAEAAGNLVGPRDFVSVRCAKRRGSTCVLAGMATDFGNMPEQKGVIRAEHGPTCMVLHPLAGSPSKTKLTWLLSIDLKGWLPKSIINQVLSQTQVDFANHLRKRLESHPASEARC

• Full Name: steroidogenic acute regulatory protein
• Calculated Molecular Weight: 285 aa, 32 kDa
• Observed Molecular Weight: 26-30 kDa
• GenBank Accession Number: BC010550
• Gene Symbol: STAR
• Gene ID (NCBI): 6770
• RRID: AB_2115832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P49675
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Steroidogenic acute regulatory protein (StAR) is a key player in acute regulation of hormone-induced steroidogenesis, by facilitating cholesterol transport from cellular stores to inner mitochondria membrane where the cholesterol was conversed to pregnenolone. StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue.

Publications

What published studies show

Anti-StAR (Steroidogenic Acute Regulatory Protein) antibody, Cat# 12225-1-AP, has accumulated 98 citations published in over 60 journals, including Science, Free Radical Biology and Medicine, Cell Death & Disease, Gut Microbes, Phytomedicine, International Journal of Molecular Sciences, Journal of Ovarian Research, and Reproduction. Associated research fields encompass reproductive endocrinology, steroidogenesis, ovarian and testicular biology, PCOS, ferroptosis, oxidative stress, mitochondrial function, and environmental toxicology.

Automatically generated from the 98 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Science; Artemisinins ameliorate polycystic ovarian syndrome by mediating LONP1-CYP11A1 interaction; Authors - Yang Liu
mouse	WB	J Adv Res; SLC7A11 as a therapeutic target to attenuate phthalates-driven testosterone level decline in mice.; Authors - Yi Zhao
mouse	WB,IF	Gut Microbes; High-fat diet-induced L-saccharopine accumulation inhibits estradiol synthesis and damages oocyte quality by disturbing mitochondrial homeostasis; Authors - Jingyi Wen
mouse	WB	Cell Death Dis; Oxidized-LDL inhibits testosterone biosynthesis by affecting mitochondrial function and the p38 MAPK/COX-2 signaling pathway in Leydig cells.; Authors - Jun Jing
mouse	WB,IF	Cell Commun Signal; Mechanistic insights into targeting APLN/APJ for ameliorating testosterone deficiency and reproductive disorders in diabetic mice.; Authors - Ke Song
rat	WB,IF	Phytomedicine; Jiao-tai-wan and its component coptisine attenuate PCOS by regulating mitochondrial cholesterol import through suppression of SIRT1 ubiquitination.; Authors - Hongzhan Wang