Tested Applications
| Positive WB detected in | pig adrenal gland tissue, human adrenal gland tissue, human testis tissue, K-562 cells, rat adrenal gland tissue, rat testis tissue, THP-1 cells, Mo7e cells |
| Positive IF-P detected in | mouse testis tissue |
| Positive IF-Fro detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67130-1-Ig targets STAR in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28698 Product name: Recombinant human STAR protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-285 aa of BC010550 Sequence: MLLATFKLCAGSSYRHMRNMKGLRQQAVMAISQELNRRALGGPTPSTWINQVRRRSSLLGSRLEETLYSDQELAYLQQGEEAMQKALGILSNQEGWKKESQQDNGDKVMSKVVPDVGKVFRLEVVVDQPMERLYEELVERMEAMGEWNPNVKEIKVLQKIGKDTFITHELAAEAAGNLVGPRDFVSVRCAKRRGSTCVLAGMATDFGNMPEQKGVIRAEHGPTCMVLHPLAGSPSKTKLTWLLSIDLKGWLPKSIINQVLSQTQVDFANHLRKRLESHPASEARC Predict reactive species |
| Full Name | steroidogenic acute regulatory protein |
| Calculated Molecular Weight | 285 aa, 32 kDa |
| Observed Molecular Weight | 28-32 kDa |
| GenBank Accession Number | BC010550 |
| Gene Symbol | STAR |
| Gene ID (NCBI) | 6770 |
| RRID | AB_2882429 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P49675 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Steroidogenic acute regulatory protein (StAR) is a key player in acute regulation of hormone-induced steroidogenesis, by facilitating cholesterol transport from cellular stores to inner mitochondria membrane where the cholesterol was conversed to pregnenolone. StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for STAR antibody 67130-1-Ig | Download protocol |
| WB protocol for STAR antibody 67130-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The STAR Monoclonal antibody (1G6E6), catalog number 67130-1-Ig, has 14 total citations published across 12 journals including Cell Reports, Small, Scientific Reports, Journal of Ethnopharmacology, International Immunopharmacology, Molecular and Cellular Endocrinology, Andrology, Peptides, Drug Design Development and Therapy, Journal of Nanobiotechnology, Toxicological Applications and Pharmacology, and Theriogenology. Research fields span steroidogenesis, Leydig cell function, spermatogenesis, polycystic ovary syndrome, reproductive endocrinology, and testicular development.
| Species | Application | Title |
|---|---|---|
J Nanobiotechnology Seminiferous tubule-inspired coaxial bioprinting-derived extracellular vesicles restore Leydig cell steroidogenesis through modulation of Wnt4/β-catenin signaling. | ||
Small Maternal Bisphenol S Exposure Impairs Testicular Development and Sperm Function in Male Offspring by Disrupting the Immune-Endocrine Network. | ||
Cell Rep High matrix stiffness triggers testosterone decline in aging males by disrupting stem Leydig cell pool homeostasis | ||
J Ethnopharmacol Saffron extract alleviates D-gal-induced late-onset hypogonadism by activating the PI3K-Akt-Nrf2 signaling pathway | ||
Drug Des Devel Ther Semaglutide Alleviates Ovary Inflammation via the AMPK/SIRT1/NF‑κB Signaling Pathway in Polycystic Ovary Syndrome Mice | ||
Int Immunopharmacol Melatonin orchestrates cAMP-PKA signaling to alleviate zearalenone-impaired the estradiol synthesis in mammal ovary. |
