STARD3NL Polyclonal antibody

• Featured Product
• KD/KO Validated

STARD3NL Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20502-1-AP

Synonyms

MENTHO, STARD3 N terminal like, STARD3 N terminal like protein, STARD3NL

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Validation Data Gallery

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  WB analysis using 20502-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20502-1-AP (STARD3NL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 20502-1-AP

  IP result of anti-STARD3NL (IP:20502-1-AP, 4ug; Detection:20502-1-AP 1:500) with HEK-293 cells lysate 3200ug.

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  IHC staining of human breast cancer using 20502-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20502-1-AP (STARD3NL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20502-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20502-1-AP (STARD3NL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 20502-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using STARD3NL antibody (20502-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: U2OS cells, HEK-293 cells, mouse kidney tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

20502-1-AP targets STARD3NL in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STARD3NL fusion protein Ag14273
• Full Name: STARD3 N-terminal like
• Calculated Molecular Weight: 234 aa, 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC005959
• Gene Symbol: STARD3NL
• Gene ID (NCBI): 83930
• RRID: AB_10694281
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB	J Cell Mol Med; STARD3NL inhibits the osteogenic differentiation by inactivating the Wnt/β-catenin pathway via binding to Annexin A2 in osteoporosis.; Authors - Yuexin Xu ; KD Validated