STARD7 Recombinant antibody

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  WB analysis using 85131-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85131-4-RR (STARD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 85131-4-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85131-4-RR (STARD7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 85131-4-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85131-4-RR (STARD7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 85131-4-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85131-4-RR (STARD7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 85131-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85131-4-RR against Human STARD7 were performed. The affinity constant is below 1 pM.

STARD7 Recombinant Antibody for WB, IHC, ELISA

Cat No. 85131-4-RR

Clone No.242760A3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

242760A3, GTT1, StAR-related lipid transfer protein 7, mitochondrial, START domain-containing protein 7

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HT-1080 cells, MCF-7 cells, K-562 cells, mouse testis tissue, rat testis tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85131-4-RR targets STARD7 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: STARD7 fusion protein Ag8224
• Full Name: StAR-related lipid transfer (START) domain containing 7
• Calculated Molecular Weight: 295 aa, 35 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC008894
• Gene Symbol: STARD7
• Gene ID (NCBI): 56910
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NQZ5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STARD7, also known as gestational trophoblastic tumor gene-1 (GTT1), is a lipid transfer protein that specifically binds and transports phosphatidylcholine (PC) between cellular membranes. It is a member of the START (Steroidogenic Acute Regulatory protein-related lipid transfer) domain-containing protein family, which is involved in intracellular lipid transport and metabolism. (PMID: 23507753)