STAT5B Recombinant monoclonal antibody, PBS Only (Capture)

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  WB analysis using 87224-2-RR (same clone as 87224-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87224-2-RR (STAT5B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87224-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 87224-2-RR (same clone as 87224-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using STAT5B antibody (87224-2-RR, Clone: 252436G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87224-2-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02926-1

  The mean STAT5B concentration was determined to be 154.7 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load, 194.7 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load, 86.8 ng/mL in A431 cell extract based on a 1.2 mg/mL extract load.

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  Cytometric bead array standard curve of MP02926-1

  Cytometric bead array standard curve of MP02926-1, STAT5B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87224-2-PBS. Detection antibody: 87224-1-PBS. Standard: 1928-46/80. Range: 19.53-5000 pg/mL.

STAT5B Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 87224-2-PBS

Clone No.252436G2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

STAT5A/B, STAT5

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Product Information

87224-2-PBS targets STAT5B as part of a matched antibody pair:

MP02926-1: 87224-2-PBS capture and 87224-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: signal transducer and activator of transcription 5B
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC065227
• Gene Symbol: STAT5B
• Gene ID (NCBI): 6777
• ENSEMBL Gene ID: ENSG00000173757
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P51692
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

STAT5B belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5B carries out a dual function: signal transduction and activation of transcription. STAT5B mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression.