STAT6 70kDa Polyclonal antibody

STAT6 70kDa Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10257-1-AP

Synonyms

D12S1644, IL 4 STAT, STAT6, STAT6B, STAT6C



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 10257-1-AP is STAT6 70kDa Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STAT6 70kDa fusion protein Ag0410
Full Name signal transducer and activator of transcription 6, interleukin-4 induced
Calculated molecular weight 100 kDa
Observed molecular weight 75-80 kDa
GenBank accession numberBC005823
Gene symbol STAT6
Gene ID (NCBI) 6778
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. STAT6 forms a homodimer or a heterodimer with a related family member. STAT6 translocates into the nucleus in response to phosphorylation. STAT6 is Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling. This poly-antibody only recognizes 70 kDa isoform of STAT6.

Protocols

Product Specific Protocols
WB protocol for STAT6 70kDa antibody 10257-1-APDownload protocol
IHC protocol for STAT6 70kDa antibody 10257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols