Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10257-1-AP targets STAT6 70kDa in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0410 Product name: Recombinant human STAT6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 368-567 aa of BC005823 Sequence: IKRCERKGTESVTEEKCAVLFSASFTLGPGKLPIQLQALSLPLVVIVHGNQDNNAKATILWYNAFSEMDRVPFVVAERVPWEKMCETLNLKFMAEVGTNRGLLPEHFLFLAQKIFNDNSLSMEAFQHRSVSWSQFNKEILLGRGFTFWQWFDGVLDLTKRCLRSYWSDRLIIGFISKQYVTSLLLNEPDGTFLLRFSDSE Predict reactive species |
| Full Name | signal transducer and activator of transcription 6, interleukin-4 induced |
| Calculated Molecular Weight | 100 kDa |
| Observed Molecular Weight | 75-80 kDa |
| GenBank Accession Number | BC005823 |
| Gene Symbol | STAT6 |
| Gene ID (NCBI) | 6778 |
| RRID | AB_10695044 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42226 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. STAT6 forms a homodimer or a heterodimer with a related family member. STAT6 translocates into the nucleus in response to phosphorylation. STAT6 is Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling. This poly-antibody only recognizes 70 kDa isoform of STAT6.
Publications
What published studies show
The STAT6 70kDa Polyclonal antibody (Cat# 10257-1-AP) has 3 citations published in Biomaterials, Pharm Biol, and Front Pharmacol. The research fields span neuroimmunology (Alzheimer's disease treatment via Treg conversion and microglial phagocytosis), hepatic fibrosis (macrophage M1 polarization inhibition), and inflammatory signaling (SHP2 downregulation).
| Species | Application | Title |
|---|---|---|
Biomaterials Engineered peripheral CD4 T cells delivery across the BBB promote intracerebral Treg conversion unleashes microglial phagocytotic activity for Alzheimer's disease treatment | ||
Pharm Biol Yiguanjian decoction inhibits macrophage M1 polarization and attenuates hepatic fibrosis induced by CCl4/2-AAF. | ||
Front Pharmacol Isovitexin Inhibits Ginkgolic Acids-Induced Inflammation Through Downregulating SHP2 Activation. |
