STAT6 Monoclonal antibody

STAT6 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

2E5B8

Cat no : 66717-1-Ig

Synonyms

D12S1644, IL 4 STAT, STAT6, STAT6B, STAT6C

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Validation Data Gallery

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  WB analysis using 66717-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66717-1-Ig (STAT6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis using 66717-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66717-1-Ig (STAT6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

66717-1-Ig targets STAT6 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: STAT6 fusion protein Ag0410
• Full Name: signal transducer and activator of transcription 6, interleukin-4 induced
• Calculated molecular weight: 100 kDa
• Observed molecular weight: 95 kDa
• GenBank accession number: BC005823
• Gene symbol: STAT6
• Gene ID (NCBI): 6778
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. STAT6 forms a homodimer or a heterodimer with a related family member. STAT6 translocates into the nucleus in response to phosphorylation. STAT6 is Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

Publications

Species	Application	Title
mouse	IF	Front Immunol; PPARγ as an E3 Ubiquitin-Ligase Impedes Phosphate-Stat6 Stability and Promotes Prostaglandins E2-Mediated Inhibition of IgE Production in Asthma.; Authors - Jia Wu
human	WB	J Cell Mol Med; Fructose-1,6-bisphosphatase aggravates oxidative stress-induced apoptosis in asthma by suppressing the Nrf2 pathway.; Authors - Jiapeng Hu
mouse	WB	Immunol Invest; Absence of TNF Leads to Alternative Activation in Peritoneal Macrophages in Experimental Listeria Monocytogenes Infection.; Authors - Xinying Li