STAT6 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82630-1-RR (same clone as 82630-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82630-1-RR (STAT6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82630-1-RR (same clone as 82630-1-PBS)

  WB result of STAT6 antibody (82630-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT6 transfected HeLa cells. This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 82630-1-RR (same clone as 82630-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82630-1-RR (STAT6 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 82630-1-RR (same clone as 82630-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82630-1-RR (STAT6 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 82630-1-RR (same clone as 82630-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82630-1-RR (STAT6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 82630-1-RR (same clone as 82630-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82630-1-RR (STAT6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 82630-1-RR (same clone as 82630-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using STAT6 antibody (82630-1-RR, Clone: 4F19 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

STAT6 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 82630-1-PBS

Clone No.4F19

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

4F19, IL-4 Stat, Signal transducer and activator of transcription 6, STAT 6, STAT6B

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Product Information

82630-1-PBS targets STAT6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: signal transducer and activator of transcription 6, interleukin-4 induced
• Calculated Molecular Weight: 94 kDa
• Observed Molecular Weight: 105-110 kDa
• GenBank Accession Number: BC075852
• Gene Symbol: STAT6
• Gene ID (NCBI): 6778
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P42226
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. STAT6 forms a homodimer or a heterodimer with a related family member. STAT6 translocates into the nucleus in response to phosphorylation. STAT6 is Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.