Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells |
| Positive IHC detected in | human colon cancer tissue, human stomach tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82630-1-RR targets STAT6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | signal transducer and activator of transcription 6, interleukin-4 induced |
| Calculated Molecular Weight | 94 kDa |
| Observed Molecular Weight | 105-110 kDa |
| GenBank Accession Number | BC075852 |
| Gene Symbol | STAT6 |
| Gene ID (NCBI) | 6778 |
| RRID | AB_3086494 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P42226 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. STAT6 forms a homodimer or a heterodimer with a related family member. STAT6 translocates into the nucleus in response to phosphorylation. STAT6 is Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for STAT6 antibody 82630-1-RR | Download protocol |
| IHC protocol for STAT6 antibody 82630-1-RR | Download protocol |
| WB protocol for STAT6 antibody 82630-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The STAT6 Recombinant monoclonal antibody (4F19), catalog number 82630-1-RR, has a total of 5 citations. These appear in Advanced Science, Cell Communication and Signaling, BMC Complementary Medicine and Therapies, Neuroreport, and Cancers. The associated research fields span renal cell carcinoma metastasis, liver cancer drug sensitivity, non-small cell lung cancer immunotherapy, optic nerve axonal regeneration, and triple-negative breast cancer glycolysis regulation.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) The FOXC2-LAMA4 Axis Orchestrates Vasculogenic Mimicry and Immunosuppressive Niche Formation to Drive Metastatic Cascade in Renal Cell Carcinoma. | ||
Cell Commun Signal Whole-gene CRISPR/cas9 library screen revealed targeting STAT6 increased the sensitivity of liver cancer to celecoxib via inhibiting arachidonic acid shunting.
| ||
BMC Complement Med Ther Qingrehuoxue formula enhances anti-PD-1 immunotherapy in NSCLC by remodeling the tumor immune microenvironment via TREM2 signaling | ||
Cancers (Basel) EIF3H Modulates Glycolysis Through LDHA Stabilization in Triple-Negative Breast Cancer. |
