STAU1 Monoclonal antibody

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  WB analysis using 61024-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 61024-1-Ig (STAU1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit liver using 61024-1-Ig

  rabbit liver tissue were subjected to SDS PAGE followed by western blot with 61024-1-Ig (STAU1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

STAU1 Monoclonal Antibody for WB, ELISA

Cat No. 61024-1-Ig

Clone No.1H1D7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit

Applications

WB, ELISA

Synonyms

Double-stranded RNA-binding protein Staufen homolog 1, STAU

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Tested Applications

• Positive WB detected in: A549 cells, rabbit liver tissue, LNCaP cells, MCF-7 cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

61024-1-Ig targets STAU1 in WB, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

• Tested Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5573

  Product name: Recombinant human STAU1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 227-577 aa of BC050432

  Sequence: GEGEGKSKKISKKNAAIAVLEELKKLPPLPAVERVKPRIKKKTKPIVKPQTSPEYGQGINPISRLAQIQQAKKEKEPEYTLLTERGLPRRREFVMQVKVGNHTAEGTGTNKKVAKRNAAENMLEILGFKVPQAQPTKPALKSEEKTPIKKPGDGRKVTFFEPGSGDENGTSNKEDEFRMPYLSHQQLPAGILPMVPEVAQAVGVSQGHHTKDFTRAAPNPAKATVTAMIARELLYGGTSPTAETILKNNISSGHVPHGPLTRPSEQLDYLSRVQGFQVEYKDFPKNNKNEFVSLINCSSQPPLISHGIGKDVESCHDMAALNILKLLSELDQQSTEMPRTGNGPMSVCGRC

• Full Name: staufen, RNA binding protein, homolog 1 (Drosophila)
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: BC050432
• Gene Symbol: STAU1
• Gene ID (NCBI): 6780
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O95793
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Staufen1 (STAU1)-mediated mRNA decay (SMD) degrades translationally active mRNAs that bind the double-stranded (ds)RNA binding protein STAU1 within their 3'-untranslated regions (3'UTRs) [PMID:21307942]. STAU1 can binds to stem structures and protect Dvl2 mRNA from degradation by associating with the secondary structure in the U1 region which is located at 5′ position of second U stretch in the U2 region [PMID:17510634]. It has a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site.