Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, HeLa cells, A549 cells |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11842-1-AP targets STBD1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2481 Product name: Recombinant human STBD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 26-358 aa of BC022301 Sequence: GPGDTGKDGDAEQEKDAPLGGAAIPGGHQSGSSGLSPGPSGQELVTKPEHLQESNGHLISKTKDLGKLQAASWRLQNPSREVCDNSREHVPSGQFPDTEAPATSETSNSRSYSEVSRNESLESPMGEWGFQKGQEISAKAATCFAEKLPSSNLLKNRAKEEMSLSDLNSQDRVDHEEWEMVPRHSSWGDVGVGGSLKAPVLNLNQGMDNGRSTLVEARGQQVHGKMERVAVMPAGSQQVSVRFQVHYVTSTDVQFIAVTGDHECLGRWNTYIPLHYNKDGFWSHSIFLPADTVVEWKFVLVENGGVTRWEECSNRFLETGHEDKVVHAWWGIH Predict reactive species |
| Full Name | starch binding domain 1 |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 35-43 kDa |
| GenBank Accession Number | BC022301 |
| Gene Symbol | STBD1 |
| Gene ID (NCBI) | 8987 |
| RRID | AB_2197523 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95210 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
STBD1 may have the capability to bind to carbohydrates. It functions as a glycogen receptor that tethers glycogen to autophagic membranes for delivery and breakdown in lysosomes. STBD1 appears molecular mass of 35-38 kDa band in mouse/rat tissues and 38-43 kDa in human tissue.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for STBD1 antibody 11842-1-AP | Download protocol |
| IP protocol for STBD1 antibody 11842-1-AP | Download protocol |
| WB protocol for STBD1 antibody 11842-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The STBD1 Polyclonal antibody (Cat# 11842-1-AP) has 24 citations. These appear in journals including Cell, Molecular Cell, Nature Communications, Redox Biology, Cell Reports, eLife, EMBO Reports, Journal of Biological Chemistry, Journal of Cell Science, Journal of Pathology, Acta Pharmacologica Sinica, and Molecular Metabolism. Research fields span glycogen metabolism and glycophagy, liver disease, Pompe disease, muscle atrophy, ischemic stroke, lipid droplet biology, and metabolic regulation.
| Species | Application | Title |
|---|---|---|
Redox Biol Txnrd2 loss in skeletal muscle causes muscle atrophy and drives leanness and obesity resistance. | ||
Redox Biol Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke | ||
Mol Cell Glucose-1-phosphate promotes compartmentalization of glycogen with the pentose phosphate pathway in CD8+ memory T cells | ||
Acta Pharmacol Sin Asiatic acid alleviates ischemic myocardial injury in mice by modulating mitophagy- and glycophagy-based energy metabolism. | ||
Cell Rep Glycogen Dynamics Drives Lipid Droplet Biogenesis during Brown Adipocyte Differentiation |
