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STBD1 Polyclonal antibody

STBD1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 11842-1-AP

Synonyms

Genethonin 1, GENEX3414, GENX 3414, starch binding domain 1, STBD1



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, HeLa cells, A549 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11842-1-AP targets STBD1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STBD1 fusion protein Ag2481
Full Name starch binding domain 1
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 35-43 kDa
GenBank Accession NumberBC022301
Gene Symbol STBD1
Gene ID (NCBI) 8987
RRIDAB_2197523
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STBD1 may have the capability to bind to carbohydrates. It functions as a glycogen receptor that tethers glycogen to autophagic membranes for delivery and breakdown in lysosomes. STBD1 appears molecular mass of 35-38 kDa band in mouse/rat tissues and 38-43 kDa in human tissue.

Protocols

Product Specific Protocols
WB protocol for STBD1 antibody 11842-1-APDownload protocol
IHC protocol for STBD1 antibody 11842-1-APDownload protocol
IP protocol for STBD1 antibody 11842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell

Glycogen accumulation and phase separation drives liver tumor initiation.

Authors - Qingxu Liu
human,mouseWB,IF

Redox Biol

Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke

Authors - Haiyun Guo
mouseWB

Acta Pharmacol Sin

Asiatic acid alleviates ischemic myocardial injury in mice by modulating mitophagy- and glycophagy-based energy metabolism.

Authors - Fan Qiu
mouseWB,IF

J Cell Sci

Stbd1 promotes glycogen clustering during endoplasmic reticulum stress and supports survival of mouse myoblasts

Authors - Andria A. Lytridou
  • KD Validated
mouseWB

Mol Endocrinol

Transgenic muscle specific Nor-1 expression regulates multiple pathways that effect adiposity, metabolism and endurance.

Authors - Pearen Michael A MA
WB,IF

J Cell Sci

Mouse Stbd1 is N-myristoylated and affects ER-mitochondria association and mitochondrial morphology.

Authors - Anthi Demetriadou