STBD1 Polyclonal antibody

• Featured Product
• KD/KO Validated

STBD1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 11842-1-AP

Synonyms

Genethonin 1, GENEX3414, GENX 3414, starch binding domain 1, STBD1

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 11842-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11842-1-AP (STBD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11842-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11842-1-AP (STBD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 11842-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11842-1-AP (STBD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 11842-1-AP

  IP result of anti-STBD1 (IP:11842-1-AP, 4ug; Detection:11842-1-AP 1:1000) with A549 cells lysate 1600ug.

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  IHC staining of human colon cancer using 11842-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11842-1-AP (STBD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11842-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11842-1-AP (STBD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, HeLa cells, A549 cells
• Positive IP detected in: A549 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 7 publications below
• IP: See 2 publications below

Product Information

11842-1-AP targets STBD1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STBD1 fusion protein Ag2481
• Full Name: starch binding domain 1
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 35-43 kDa
• GenBank Accession Number: BC022301
• Gene Symbol: STBD1
• Gene ID (NCBI): 8987
• RRID: AB_2197523
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STBD1 may have the capability to bind to carbohydrates. It functions as a glycogen receptor that tethers glycogen to autophagic membranes for delivery and breakdown in lysosomes. STBD1 appears molecular mass of 35-38 kDa band in mouse/rat tissues and 38-43 kDa in human tissue.

Publications

Species	Application	Title
mouse	IF	Cell; Glycogen accumulation and phase separation drives liver tumor initiation.; Authors - Qingxu Liu
human,mouse	WB,IF	Redox Biol; Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke; Authors - Haiyun Guo
mouse	WB	Acta Pharmacol Sin; Asiatic acid alleviates ischemic myocardial injury in mice by modulating mitophagy- and glycophagy-based energy metabolism.; Authors - Fan Qiu
mouse	WB,IF	J Cell Sci; Stbd1 promotes glycogen clustering during endoplasmic reticulum stress and supports survival of mouse myoblasts; Authors - Andria A. Lytridou ; KD Validated
mouse	WB	Mol Endocrinol; Transgenic muscle specific Nor-1 expression regulates multiple pathways that effect adiposity, metabolism and endurance.; Authors - Pearen Michael A MA
	WB,IF	J Cell Sci; Mouse Stbd1 is N-myristoylated and affects ER-mitochondria association and mitochondrial morphology.; Authors - Anthi Demetriadou