STEAP2 Polyclonal antibody

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STEAP2 Polyclonal Antibody for WB, ELISA

Cat No. 20201-1-AP Copied!

Publications (5)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

STEAP2-Specific, EC:1.16.1.-, Metalloreductase STEAP2, PCANAP1, Prostate cancer-associated protein 1

• 

  WB analysis of HEK-293 using 20201-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 20201-1-AP

  WB result of STEAP2-Specific antibody (20201-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-STEAP2-Specific transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of DU 145 using 20201-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of fetal human brain using 20201-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 20201-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 20201-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, DU 145 cells, fetal human brain tissue, mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20201-1-AP targets STEAP2 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: six transmembrane epithelial antigen of the prostate 2
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 48-52 kDa
• GenBank Accession Number: NM_152999
• Gene Symbol: STEAP2
• Gene ID (NCBI): 261729
• RRID: AB_10665556
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NFT2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STEAP2, also named as PCANAP1 and STAMP1, belongs to the STEAP family. It is a metalloreductase that has the ability to reduce both Fe3+ to Fe2+ and Cu2+ to Cu1+. STEAP2 uses NAD+ as acceptor. The antibody is specific to STEAP2.

Publications

What published studies show

The STEAP2 antibody (Cat# 20201-1-AP) has 5 total citations. These appear in Cell Death & Disease, Molecular Cancer Research, Cancer Biology & Therapy, Journal of Cellular and Molecular Medicine, and Experimental Cell Research. Research fields span papillary thyroid cancer (m6A/Hedgehog signaling), prostate cancer (androgen response/extracellular vesicles), osteosarcoma (EMT/PI3K-AKT-mTOR), idiopathic pulmonary fibrosis (copper metabolism), and intracellular protein trafficking. All studies used WB in human or mouse samples.

Automatically generated from the 5 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Death Dis; METTL3-mediated m6A modification of STEAP2 mRNA inhibits papillary thyroid cancer progress by blocking the Hedgehog signaling pathway and epithelial-to-mesenchymal transition.; Authors - Yue Zhu ; KD Validated
human	WB	Mol Cancer Res; Exploring STEAP1 Expression in Prostate Cancer Cells in Response to Androgen Deprivation and in Small Extracellular Vesicles; Authors - Candice L Bizzaro
human	WB	Cancer Biol Ther; STEAP2 promotes osteosarcoma progression by inducing epithelial–mesenchymal transition via the PI3K/AKT/mTOR signaling pathway and is regulated by EFEMP2; Authors - Dong Zhang ; KD Validated
mouse	WB	J Cell Mol Med; Unveiling the role of copper metabolism and STEAP2 in idiopathic pulmonary fibrosis molecular landscape; Authors - Yajun Wang ; KD Validated
human	WB	Exp Cell Res; Membrane cholesterol modulates STEAP2 conformation during dynamic intracellular trafficking processes leading to broad subcellular distribution.; Authors - Haruki Hasegawa