|Positive WB detected in||HeLa cells, HEK-293 cells, Jurkat cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||mouse spleen tissue, human brain tissue, human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21192-1-AP is STIM2 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||STIM2 fusion protein Ag15530|
|Full Name||stromal interaction molecule 2|
|Calculated molecular weight||754 aa, 85 kDa|
|Observed molecular weight||85-115 kDa|
|GenBank accession number||BC146661|
|Gene ID (NCBI)||57620|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
STIM2, also named as KIAA1482, functions as a highly sensitive Ca2+ sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca2+-influx. Regulates basal cytosolic and endoplasmic reticulum Ca2+ concentrations. Upon mild variations of the endoplasmic reticulum Ca2+ concentration, STIM2 translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca2+ release-activated Ca2+ (CRAC) channels ORAI1, ORAI2 and ORAI3. STIM2 may inhibit STIM1-mediated Ca2+ influx. STIM2 has 2 isoforms 105kd and 115kd with Differential levels of phosphorylation.
A short isoform of STIM1 confers frequency-dependent synaptic enhancement.
Exp Ther Med
Resveratrol sensitizes A549 cells to irradiation damage via suppression of store-operated calcium entry with Orai1 and STIM1 downregulation.
Biochem Biophys Res Commun
STIM1 and STIM2 differently regulate endogenous Ca(2+) entry and promote TGF-β-induced EMT in breast cancer cells.
RGS10 physically and functionally interacts with STIM2 and requires store-operated calcium entry to regulate proinflammatory gene expression in microglia.
STIM1 negatively regulates Ca2+ release from the sarcoplasmic reticulum in skeletal myotubes.