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  • KD/KO Validated

STIM2 Polyclonal antibody

STIM2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21192-1-AP

Synonyms

KIAA1482, STIM2, stromal interaction molecule 2



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inmouse spleen tissue, human brain tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21192-1-AP is STIM2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STIM2 fusion protein Ag15530
Full Name stromal interaction molecule 2
Calculated molecular weight 754 aa, 85 kDa
Observed molecular weight 85-115 kDa
GenBank accession numberBC146661
Gene symbol STIM2
Gene ID (NCBI) 57620
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STIM2, also named as KIAA1482, functions as a highly sensitive Ca2+ sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca2+-influx. Regulates basal cytosolic and endoplasmic reticulum Ca2+ concentrations. Upon mild variations of the endoplasmic reticulum Ca2+ concentration, STIM2 translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca2+ release-activated Ca2+ (CRAC) channels ORAI1, ORAI2 and ORAI3. STIM2 may inhibit STIM1-mediated Ca2+ influx. STIM2 has 2 isoforms 105kd and 115kd with Differential levels of phosphorylation.

Protocols

Product Specific Protocols
WB protocol for STIM2 antibody 21192-1-APDownload protocol
IHC protocol for STIM2 antibody 21192-1-APDownload protocol
IP protocol for STIM2 antibody 21192-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIP

Cell Rep

PMID: 31801093

Authors - Benjamin D Stein
humanWB

Cell Rep

A short isoform of STIM1 confers frequency-dependent synaptic enhancement.

Authors - Girish Ramesh
  • KO Validated
humanWB

Exp Ther Med

Resveratrol sensitizes A549 cells to irradiation damage via suppression of store-operated calcium entry with Orai1 and STIM1 downregulation.

Authors - Wu Lele
humanWB

Biochem Biophys Res Commun

STIM1 and STIM2 differently regulate endogenous Ca(2+) entry and promote TGF-β-induced EMT in breast cancer cells.

Authors - Siheng Zhang
mouseWB

Cell Signal

RGS10 physically and functionally interacts with STIM2 and requires store-operated calcium entry to regulate proinflammatory gene expression in microglia.

Authors - Menbere Wendimu
  • KD Validated

Biochem J

STIM1 negatively regulates Ca2+ release from the sarcoplasmic reticulum in skeletal myotubes.

Authors - Lee Keon Jin KJ