Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | mouse spleen tissue, human brain tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21192-1-AP targets STIM2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15530 Product name: Recombinant human STIM2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 525-674 aa of BC146661 Sequence: EKICGFQIAHNSGLPSLTSSLYSDHSWVVMPRVSIPPYPIAGGVDDLDEDTPPIVSQFPGTMAKPPGSLARSSSLCRSRRSIVPSSPQPQRAQLAPHAPHPSHPRHPHHPQHTPHSLPSPDPDILSVSSCPALYRNEEEEEAIYFSAEKQ Predict reactive species |
| Full Name | stromal interaction molecule 2 |
| Calculated Molecular Weight | 754 aa, 85 kDa |
| Observed Molecular Weight | 85-115 kDa |
| GenBank Accession Number | BC146661 |
| Gene Symbol | STIM2 |
| Gene ID (NCBI) | 57620 |
| RRID | AB_10734322 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P246 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
STIM2, also named as KIAA1482, functions as a highly sensitive Ca2+ sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca2+-influx. Regulates basal cytosolic and endoplasmic reticulum Ca2+ concentrations. Upon mild variations of the endoplasmic reticulum Ca2+ concentration, STIM2 translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca2+ release-activated Ca2+ (CRAC) channels ORAI1, ORAI2 and ORAI3. STIM2 may inhibit STIM1-mediated Ca2+ influx. STIM2 has 2 isoforms 105kd and 115kd with Differential levels of phosphorylation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for STIM2 antibody 21192-1-AP | Download protocol |
| IHC protocol for STIM2 antibody 21192-1-AP | Download protocol |
| IP protocol for STIM2 antibody 21192-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The STIM1 antibody (Cat# 21192-1-AP) has 18 total citations published in journals including Cell Research, Nature Communications, Cell Reports, Cell Molecular Life Sciences, Neurobiology of Disease, European Journal of Pharmacology, American Journal of Respiratory Cell and Molecular Biology, Oncotarget, Cell Signaling, Biochemical Journal, Neurotoxicology, and others. Research fields span calcium signaling, store-operated calcium entry, cancer biology, neuroscience, cardiovascular disease, pulmonary hypertension, epithelial-mesenchymal transition, and metabolic regulation.
| Species | Application | Title |
|---|---|---|
Cell Res Cholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate.
| ||
Nat Commun A small molecule VDAC ligand inhibits ERAD and induces selective cancer cell death via disruption of calcium homeostasis. | ||
J Orthop Translat Tibial cortex transverse transport regulates Orai1/STIM1-mediated NO release and improve the migration and proliferation of vessels via increasing osteopontin expression | ||
Cell Rep A short isoform of STIM1 confers frequency-dependent synaptic enhancement.
| ||
Cell Rep Deficiency of ER Ca2+ sensor STIM1 in AgRP neurons confers protection against dietary obesity. |
