STIM2 Polyclonal antibody

• Featured Product
• KD/KO Validated

STIM2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 21192-1-AP

Synonyms

KIAA1482, STIM2, stromal interaction molecule 2

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Validation Data Gallery

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  WB analysis using 21192-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21192-1-AP (STIM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21192-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21192-1-AP (STIM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 21192-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21192-1-AP (STIM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 21192-1-AP

  IP result of anti-STIM2 (IP:21192-1-AP, 3ug; Detection:21192-1-AP 1:500) with HEK-293 cells lysate 2000ug.

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  IHC staining of mouse spleen using 21192-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 21192-1-AP (STIM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 21192-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21192-1-AP (STIM2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 21192-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21192-1-AP (STIM2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human brain using 21192-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21192-1-AP (STIM2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 21192-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21192-1-AP (STIM2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 21192-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 21192-1-AP (STIM2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 21192-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 21192-1-AP (STIM2 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: mouse spleen tissue, human brain tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 8 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

The immunogen of 21192-1-AP is STIM2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STIM2 fusion protein Ag15530
• Full Name: stromal interaction molecule 2
• Calculated Molecular Weight: 754 aa, 85 kDa
• Observed Molecular Weight: 85-115 kDa
• GenBank Accession Number: BC146661
• Gene Symbol: STIM2
• Gene ID (NCBI): 57620
• RRID: AB_10734322
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STIM2, also named as KIAA1482, functions as a highly sensitive Ca2+ sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca2+-influx. Regulates basal cytosolic and endoplasmic reticulum Ca2+ concentrations. Upon mild variations of the endoplasmic reticulum Ca2+ concentration, STIM2 translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca2+ release-activated Ca2+ (CRAC) channels ORAI1, ORAI2 and ORAI3. STIM2 may inhibit STIM1-mediated Ca2+ influx. STIM2 has 2 isoforms 105kd and 115kd with Differential levels of phosphorylation.

Publications

Species	Application	Title
mouse	WB	Cell Res; Cholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate.; Authors - Ao Hu ; KO Validated
human,mouse	WB,IF	Cell Mol Life Sci; STIM2 regulates NMDA receptor endocytosis that is induced by short-term NMDA receptor overactivation in cortical neurons; Authors - Karolina Serwach ; KD Validated
human	WB	Oncotarget; The calcium channel proteins ORAI3 and STIM1 mediate TGF-β induced Snai1 expression.; Authors - Atrayee Bhattacharya ; KD Validated
mouse	WB	Neurobiol Dis; Inhibiting store-operated calcium entry attenuates white matter secondary degeneration following SCI.; Authors - Ben C Orem
		Biochem J; STIM1 negatively regulates Ca2+ release from the sarcoplasmic reticulum in skeletal myotubes.; Authors - Lee Keon Jin KJ
mouse	WB	Cell Signal; RGS10 physically and functionally interacts with STIM2 and requires store-operated calcium entry to regulate proinflammatory gene expression in microglia.; Authors - Menbere Wendimu ; KD Validated