STMN2 Recombinant monoclonal antibody, PBS Only

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  IF Staining of SH-SY5Y using 82925-3-RR (same clone as 82925-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using STMN2 antibody (82925-3-RR, Clone: 230110G10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82925-3-PBS in a different storage buffer formulation.

• 

  FC experiment of SH-SY5Y using 82925-3-RR (same clone as 82925-3-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug STMN2 Recombinant antibody (82925-3-RR, Clone:230110G10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82925-3-PBS in a different storage buffer formulation.

• 

  FC experiment of SH-SY5Y using 82925-3-PBS

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug STMN2 Recombinant antibody, PBS Only (82925-3-PBS, Clone:230110G10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

STMN2 Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Cat No. 82925-3-PBS

Clone No.230110G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

230110G10, SCG 10, SCG10, SCGN 10, SCGN10

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Tested Applications

• Positive FC (Intra) detected in: SH-SY5Y cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82925-3-PBS targets STMN2 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag0929

  Product name: Recombinant human STMN2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-179 aa of BC006302

  Sequence: MAKTAMAYKEKMKELSMLSLICSCFYPEPRNINIYTYDDMEVKQINKRASGQAFELILKPPSPISEAPRTLASPKKKDLSLEEIQKKLEAAEERRKSQEAQVLKQLAEKREHEREVLQKALEENNNFSKMAEEKLILKMEQIKENREANLAAIIERLQEKERHAAEVRRNKELQVELSG

• Full Name: stathmin-like 2
• Calculated Molecular Weight: 21 kDa
• GenBank Accession Number: BC006302
• Gene Symbol: STMN2
• Gene ID (NCBI): 11075
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q93045
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Stathmin-2 (encoded by STMN2), a member of the stathmin family of proteins, encodes a phosphoprotein that has an important role in microtubule dynamics. The STMN2 protein promotes microtubule dynamics in axonal growth cones , and is thought to play an important role in neurite outgrowth. STMN2 protein is localized in both Golgi and cytosolic compartments. Its expression appears modulated in osteoblasts by nerve growth factor, dexamethasone or RhoA kinase inhibitor Y-27632 which are known effectors of osteogenesis. (PMID: 33841129, PMID: 18611392)