STOML2 Monoclonal antibody, PBS Only

• 

  WB analysis of pig brain using 60052-1-Ig (same clone as 60052-1-PBS)

  Pig, rat, and mouse brain tissues were subjected to SDS PAGE followed by western blot with 60052-1-Ig (STOML2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 60052-1-Ig (same clone as 60052-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60052-1-Ig (STOML2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 60052-1-Ig (same clone as 60052-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60052-1-Ig (STOML2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 60052-1-Ig (same clone as 60052-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60052-1-Ig (STOML2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 60052-1-Ig (same clone as 60052-1-PBS)

  IP result of anti-STOML2 (IP:60052-1-Ig, 4ug; Detection:60052-1-Ig 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 60052-1-Ig (same clone as 60052-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60052-1-Ig (STOML2 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 60052-1-Ig (same clone as 60052-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60052-1-Ig (STOML2 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human endometrial cancer using 60052-1-Ig (same clone as 60052-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60052-1-Ig (STOML2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human endometrial cancer using 60052-1-Ig (same clone as 60052-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60052-1-Ig (STOML2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 60052-1-Ig (same clone as 60052-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using STOML2 antibody (60052-1-Ig, Clone: 1A2E9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

STOML2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 60052-1-PBS

Clone No.1A2E9

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

1A2E9, EPB72 like protein 2, EPB72-like protein 2, Paraprotein target 7, Paratarg-7

---

Product Information

60052-1-PBS targets STOML2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0363

  Product name: Recombinant human STOML2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 82-356 aa of BC002442

  Sequence: IVINVPEQSAVTLDNVTLQIDGVLYLRIMDPYKASYGVEDPEYAVTQLAQTTMRSELGKLSLDKVFRERESLNASIVDAINQAADCWGIRCLRYEIKDIHVPPRVKESMQMQVEAERRKRATVLESEGTRESAINVAEGKKQAQILASEAEKAEQINQAAGEASAVLAKAKAKAEAIRILAAALTQHNGDAAASLTVAEQYVSAFSKLAKDSNTILLPSNPGDVTSMVAQAMGVYGALTKAPVPGTPDSLSSGSSRDVQGTDASLDEELDRVKMS

• Full Name: stomatin (EPB72)-like 2
• Calculated Molecular Weight: 356 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC002442
• Gene Symbol: STOML2
• Gene ID (NCBI): 30968
• RRID: AB_2271235
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UJZ1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Human stomatin (band 7.2b) is a 31-kDa erythrocyte membrane protein of unknown function but implicated in the control of ion channel permeability, mechanoreception, and lipid domain organization. Stomatin (EPB72)-like 2 (STOML2, synonyms: SLP-2, HSPC108) is a 38.5-kDa protein that is overall approximately 20% similar to human stomatin. STOML2 is also present in mature human erythrocytes , but lacks a characteristic NH(2)-terminal hydrophobic domain found in other stomatin homologues. STOML2 may link stomatin or other integral membrane proteins to the peripheral cytoskeleton and thereby play a role in regulating ion channel conductances or the organization of sphingolipid and cholesterol-rich lipid rafts.